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ZFIN ID: ZDB-FIG-141016-22
Alexander et al., 1999 - casanova plays an early and essential role in endoderm formation in zebrafish. Developmental Biology   215(2):343-357 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to 90%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage: Shield

Fig. 3 The endoderm is defective in cas mutants from the onset of gastrulation. axial (A–D), gata5 (E, F), and fkd2 (G, H) expression in wild-type (A, C, E, G) and cas (B, D, F, H) mutant embryos at shield (A, B), 80% epiboly (C–F), and 90% epiboly (G, H) stages. Soon after the onset of gastrulation (A) axial is expressed in the embryonic shield and endodermal precursors located throughout the hypoblast. Endodermal axial expression is absent in cas mutants, while expression in the embryonic shield is normal (B); the few axial-expressing cells just outside the shield in the cas mutant are likely notochord precursors that have not yet completed dorsal convergence. At midgastrulation wild-type embryos express axial in the endodermal precursors and the prechordal plate and notochord (C); no endodermal expression of axial is seen in cas mutants (D). gata5 expression also identifies endodermal precursors within the hypoblast of wild-type (E) but not cas mutant (F) embryos. gata5 expression in the anterior lateral mesoderm precursors and YSL, which is out of focus (E), appears normal in cas mutants (F). fkd2 is expressed in endodermal precursors in wild-type embryos, as well as in the YSL and axial mesoderm (G). cas mutants specifically lack endodermal fkd2 expression (H). (A, B) Animal pole views; (C, D) left lateral views, anterior to the top; (E–H) dorsal views, anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxa2 WT standard conditions Shield presumptive endoderm ISH
Shield shield ISH
75%-epiboly axial chorda mesoderm ISH
75%-epiboly endoderm ISH
75%-epiboly prechordal plate ISH
sox32ta56/ta56 standard conditions Shield Not Detected presumptive endoderm ISH
Shield shield ISH
75%-epiboly axial chorda mesoderm ISH
75%-epiboly Not Detected endoderm ISH
75%-epiboly prechordal plate ISH
foxa3 WT standard conditions 90%-epiboly axial mesoderm ISH
90%-epiboly endoderm ISH
90%-epiboly yolk syncytial layer ISH
sox32ta56/ta56 standard conditions 90%-epiboly axial mesoderm ISH
90%-epiboly Not Detected endoderm ISH
90%-epiboly yolk syncytial layer ISH
gata5 WT standard conditions 75%-epiboly endoderm ISH
75%-epiboly mesoderm ISH
75%-epiboly yolk syncytial layer ISH
sox32ta56/ta56 standard conditions 75%-epiboly Not Detected endoderm ISH
75%-epiboly mesoderm ISH
75%-epiboly yolk syncytial layer ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox32ta56/ta56 standard conditions Shield endoderm absent, abnormal
Shield endoderm formation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 215(2), Alexander, J., Rothenberg, M., Henry, G.L., and Stainier, D.Y.R., casanova plays an early and essential role in endoderm formation in zebrafish, 343-357, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.