FIGURE

Fig. 6

ID

ZDB-FIG-071120-6

Publication

Peterkin et al., 2007 - Redundancy and evolution of GATA factor requirements in development of the myocardium

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Fig. 6

Cardiac induction requires GATA activity in zebrafish. Flat mounts: dorsal views. (A) nkx2.5 expression at 5 somites requires GATA5 and redundantly GATA4 and GATA6. The loss of GATA4 and/or GATA6 alone has little effect on the initiation of nkx2.5. (B) GATA4 expression at 5 somites requires GATA5 and redundantly GATA6. GATA4 is also required to maintain its own expression (B). Hrt/tbx20 expression is lost in the GATA5 and 6 double morphants (C) as is Tbx5 expression at 10 somites (D). (E) nkx2.7 expression remains unchanged in embryos injected with GATA5 and 6 morpholino. Note that GATA5 and 6 double morphants knock out GATA4 expression and therefore represent a triple knockdown. The black bracket indicates the cardiac expression.

Expression Data

Genes:	egr2b gata4 myod1 nkx2.5 nkx2.7 tbx5a tbx20
Fish:	WT WT + MO1-gata5 WT + MO1-gata6 WT + MO3-gata4 WT + MO1-gata5 + MO1-gata6 WT + MO1-gata5 + MO3-gata4 WT + MO1-gata6 + MO3-gata4
Knockdown Reagents:	MO1-gata5 MO1-gata6 MO3-gata4
Anatomical Terms:	heart primordium presumptive rhombomere 3 presumptive rhombomere 5 somite
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 311(2), Peterkin, T., Gibson, A., and Patient, R., Redundancy and evolution of GATA factor requirements in development of the myocardium, 623-635, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.