ZFIN ID: ZDB-FIG-071120-6
Peterkin et al., 2007 - Redundancy and evolution of GATA factor requirements in development of the myocardium. Developmental Biology   311(2):623-635 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to 10-13 somites

Fig. 6 Cardiac induction requires GATA activity in zebrafish. Flat mounts: dorsal views. (A) nkx2.5 expression at 5 somites requires GATA5 and redundantly GATA4 and GATA6. The loss of GATA4 and/or GATA6 alone has little effect on the initiation of nkx2.5. (B) GATA4 expression at 5 somites requires GATA5 and redundantly GATA6. GATA4 is also required to maintain its own expression (B). Hrt/tbx20 expression is lost in the GATA5 and 6 double morphants (C) as is Tbx5 expression at 10 somites (D). (E) nkx2.7 expression remains unchanged in embryos injected with GATA5 and 6 morpholino. Note that GATA5 and 6 double morphants knock out GATA4 expression and therefore represent a triple knockdown. The black bracket indicates the cardiac expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b WT standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
WT + MO1-gata5 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
WT + MO1-gata5 + MO3-gata4 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
WT + MO1-gata6 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
WT + MO1-gata6 + MO3-gata4 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
WT + MO3-gata4 standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
gata4 WT standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata5 standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites Not Detected heart primordium ISH
WT + MO1-gata5 + MO3-gata4 standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata6 standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata6 + MO3-gata4 standard conditions 5-9 somites heart primordium ISH
WT + MO3-gata4 standard conditions 5-9 somites heart primordium ISH
myod1 WT standard conditions 5-9 somites somite ISH
WT + MO1-gata5 standard conditions 5-9 somites somite ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites somite ISH
WT + MO1-gata5 + MO3-gata4 standard conditions 5-9 somites somite ISH
WT + MO1-gata6 standard conditions 5-9 somites somite ISH
WT + MO1-gata6 + MO3-gata4 standard conditions 5-9 somites somite ISH
WT + MO3-gata4 standard conditions 5-9 somites somite ISH
nkx2.5 WT standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata5 standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites Not Detected heart primordium ISH
WT + MO1-gata5 + MO3-gata4 standard conditions 5-9 somites Not Detected heart primordium ISH
WT + MO1-gata6 standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata6 + MO3-gata4 standard conditions 5-9 somites heart primordium ISH
WT + MO3-gata4 standard conditions 5-9 somites heart primordium ISH
nkx2.7 WT standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites heart primordium ISH
tbx5a WT standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 10-13 somites Not Detected heart primordium ISH
tbx20 WT standard conditions 5-9 somites heart primordium ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 5-9 somites Not Detected heart primordium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 311(2), Peterkin, T., Gibson, A., and Patient, R., Redundancy and evolution of GATA factor requirements in development of the myocardium, 623-635, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.