ZFIN ID: ZDB-FIG-071120-5
Peterkin et al., 2007 - Redundancy and evolution of GATA factor requirements in development of the myocardium. Developmental Biology   311(2):623-635 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 5 Expression of nkx2.5 and GATA4 in zebrafish morphants of GATA4, 5 and/or 6 separately and in combination. Flat mounts, dorsal views (A) A, anterior; P, posterior; D, dorsal; V, ventral. (B) As seen at 26 hpf (Fig. 4), GATA4 morphants at 10 somites showed normal expression of nkx2.5, whereas GATA6, and more severely GATA5, morphants showed a reduction in expression. In combination morpholino injections, down regulation was exacerbated in each case, as seen at 26 hpf (Fig. 4), with expression being completely absent in embryos depleted for both GATA5 + 6. (C) Loss of GATA5 reduces the expression of GATA4 and there is a complete loss of GATA4 expression when GATA5 and 6 are depleted in combination (G5 + G6MO). The black brackets indicate the cardiac expression. Krox20 and MyoD are stained in red and were used as landmarks. Krox20 is expressed in rhombomeres 3 and 5, MyoD was used to confirm the number of somites in the embryo.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
egr2b WT standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
WT + MO1-gata5 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
WT + MO1-gata5 + MO3-gata4 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
WT + MO1-gata6 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
WT + MO1-gata6 + MO3-gata4 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
WT + MO3-gata4 standard conditions 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
gata4 WT standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata5 standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 10-13 somites Not Detected heart primordium ISH
WT + MO1-gata5 + MO3-gata4 standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata6 standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata6 + MO3-gata4 standard conditions 10-13 somites heart primordium ISH
WT + MO3-gata4 standard conditions 10-13 somites heart primordium ISH
myod1 WT standard conditions 10-13 somites somite ISH
WT + MO1-gata5 standard conditions 10-13 somites somite ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 10-13 somites somite ISH
WT + MO1-gata5 + MO3-gata4 standard conditions 10-13 somites somite ISH
WT + MO1-gata6 standard conditions 10-13 somites somite ISH
WT + MO1-gata6 + MO3-gata4 standard conditions 10-13 somites somite ISH
WT + MO3-gata4 standard conditions 10-13 somites somite ISH
nkx2.5 WT standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata5 standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata5 + MO1-gata6 standard conditions 10-13 somites Not Detected heart primordium ISH
WT + MO1-gata5 + MO3-gata4 standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata6 standard conditions 10-13 somites heart primordium ISH
WT + MO1-gata6 + MO3-gata4 standard conditions 10-13 somites heart primordium ISH
WT + MO3-gata4 standard conditions 10-13 somites heart primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gata5 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO1-gata5 + MO1-gata6 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO1-gata5 + MO3-gata4 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO1-gata6 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO1-gata6 + MO3-gata4 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO3-gata4 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 311(2), Peterkin, T., Gibson, A., and Patient, R., Redundancy and evolution of GATA factor requirements in development of the myocardium, 623-635, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.