ZFIN ID: ZDB-FIG-071120-4
Peterkin et al., 2007 - Redundancy and evolution of GATA factor requirements in development of the myocardium. Developmental Biology   311(2):623-635 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4 GATA5 and 6 are essential but GATA4 is redundant in zebrafish myocardium. Zebrafish embryos were injected with GATA4, 5 and/or 6 morpholinos individually or in combination and analysed by whole mount in situ hybridisation at 26 hpf for the expression of Nkx2.5 (A, B), vmhc (C, D) and cmlc2 (E, F). Anterior views: red brackets and arrowheads identify the cardiac expression. Embryos were classified into unaffected (type +), downregulated (type -) and absent (type --) for expression of nkx2.5 (B), vmhc (D) and cmlc2 (F) and graphically represented. The effect on all three cardiac genes studied was similar for each injection. Depletion of GATA4 alone had little impact whereas loss of GATA5 or 6 caused a reduction in the expression of all three. Combinatorial ablation of GATA4 + 5 and GATA4 + 6 caused a further reduction in expression compared to the ablations of GATA5 or GATA6 alone thus demonstrating functional redundancy for GATA4 in the expression of these cardiac genes. GATA5 + 6 ablation resulted in complete loss of expression demonstrating an additive effect for these two GATAs.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh7 WT standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 + MO1-gata6 standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 + MO3-gata4 standard conditions Prim-5 heart tube ISH
WT + MO1-gata6 standard conditions Prim-5 heart tube ISH
WT + MO1-gata6 + MO3-gata4 standard conditions Prim-5 heart tube ISH
WT + MO3-gata4 standard conditions Prim-5 heart tube ISH
myl7 WT standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 + MO1-gata6 standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 + MO3-gata4 standard conditions Prim-5 heart tube ISH
WT + MO1-gata6 standard conditions Prim-5 heart tube ISH
WT + MO1-gata6 + MO3-gata4 standard conditions Prim-5 heart tube ISH
WT + MO3-gata4 standard conditions Prim-5 heart tube ISH
nkx2.5 WT standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 + MO1-gata6 standard conditions Prim-5 heart tube ISH
WT + MO1-gata5 + MO3-gata4 standard conditions Prim-5 heart tube ISH
WT + MO1-gata6 standard conditions Prim-5 heart tube ISH
WT + MO1-gata6 + MO3-gata4 standard conditions Prim-5 heart tube ISH
WT + MO3-gata4 standard conditions Prim-5 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gata5 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO1-gata5 + MO1-gata6 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO1-gata5 + MO3-gata4 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO1-gata6 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO1-gata6 + MO3-gata4 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Prim-5 heart tube malformed, abnormal
WT + MO3-gata4 standard conditions Prim-5 embryonic heart tube development disrupted, abnormal
Prim-5 heart morphogenesis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 311(2), Peterkin, T., Gibson, A., and Patient, R., Redundancy and evolution of GATA factor requirements in development of the myocardium, 623-635, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.