ZFIN ID: ZDB-FIG-070620-23
Hinits et al., 2007 - Mef2s are required for thick filament formation in nascent muscle fibres. Development (Cambridge, England)   134(13):2511-2519 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 5 Mef2 controls a transcriptional module involved in thick filament assembly. In situ hybridisation for mRNAs encoding sarcomeric components in 24-hpf control or mef2d/c MO-injected zebrafish embryos. In each panel, cryosections of wholemount embryos, dorsal up, are to the left and flatmounts, anterior up and dorsal to right, are to the right. (A-D) Embryonic fast myhz1 (B), mylz2 (C, 22s) and smbpc (D) mRNAs are grossly downregulated in morphants. Slow fibre-specific smyhc1 mRNA (A) remains abundant. (E-G) mRNA for thin filament-related genes detected with probes to acta1 (E, 21s) or tnnc (G) are unaltered in morphants, whereas tpma (F) is upregulated. Note the lack of change in nuclear Pax3/7 protein (F, brown). (H) mRNA for the myosin chaperone protein hsp90a is also upregulated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
actc1b WT control 20-25 somites skeletal muscle cell ISH
WT + MO3-mef2d,mef2ca standard conditions 20-25 somites skeletal muscle cell ISH
hsp90aa1.1 WT control Prim-5 skeletal muscle cell ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 skeletal muscle cell ISH
mybpc1 WT control Prim-5 slow muscle cell ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 Not Detected slow muscle cell ISH
myhz1.1 WT control Prim-5 fast muscle cell ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 Not Detected fast muscle cell ISH
mylpfa WT control 20-25 somites fast muscle cell ISH
WT + MO3-mef2d,mef2ca standard conditions 20-25 somites Not Detected fast muscle cell ISH
smyhc1 WT control Prim-5 slow muscle cell ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 slow muscle cell ISH
tnnc2 WT control Prim-5 skeletal muscle cell ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 skeletal muscle cell ISH
tpma WT control Prim-5 skeletal muscle cell ISH
WT + MO3-mef2d,mef2ca standard conditions Prim-5 skeletal muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development