FIGURE

Fig. 3

ID

ZDB-FIG-070620-21

Publication

Hinits et al., 2007 - Mef2s are required for thick filament formation in nascent muscle fibres

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Fig. 3

Slow muscle myofibril defects correlate with the extent of Mef2 depletion. Zebrafish embryos in bright field (A,B,F,G), after immunodetection of all MyHC (A), slow MyHC (C-E) or Mef2 (E) or in situ mRNA hybridisation (B,F, dorsal flatmounts; G, lateral flatmount). (A,B) Slow muscle differentiation at 15 somites is unaffected by mef2d/c MO (A, somites 6-10). (C,D) Immature slow fibres in mef2d/c morphant at 24 hpf (D) are of comparable maturity to nascent fibres in a 20-hpf control embryo at the same rostrocaudal position (C). (E) Injection of mef2d MO into a hoo^tn213 heterozygote cross yielded three phenotypes at the frequencies shown. Putative heterozygotes have diminished Mef2 in both slow (superficial slice) and fast (medial slice) fibres and thinner slow myofibril bundles. Putative mutants contained no Mef2 and had immature slow fibres. (F,G) mef2d/c morphants show persistence of prdm1 in adaxial cells (F, bracket) and loss of eng2a expression (G). Scale bars: 20 µm.

Expression Data

Genes:	en2a prdm1a smyhc1
Fish:	WT WT + MO3-mef2d,mef2ca
Knockdown Reagent:	MO3-mef2d,mef2ca
Anatomical Terms:	adaxial cell slow muscle cell
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mef2ca^tn213/+ + MO1-mef2d mef2ca^tn213/tn213 + MO1-mef2d WT + MO3-mef2d,mef2ca
Knockdown Reagents:	MO1-mef2d MO3-mef2d,mef2ca
Observed In:	myofibril assembly slow muscle cell slow muscle cell myofibril
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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