ZFIN ID: ZDB-FIG-051220-28
Chen et al., 2005 - Loss of function of def selectively up-regulates {Delta}113p53 expression to arrest expansion growth of digestive organs in zebrafish. Genes & Development   19(23):2900-2911 Full text @ Genes & Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Pec-fin to Day 5

Fig. 6 Cell proliferation-related but not proapoptotic-related p53 response genes are up-regulated in the defhi429 mutant. (A) RT-PCR analysis revealed that the p53 MO created an aberrant product with a larger size resulting from insertion of 89 bp from the intron 5. This 89-bp insertion introduced an early stop codon in the p53 mRNA. (MW) Molecular size marker; (lane 1) 0.5 mM p53 MO; (lane 2) standard control MO (SC-MO, human β-globin antisense morpholinos); (lane 3) 1.0 mM p53 MO; (red arrow) wild type p53; (black arrow) misspliced p53. (B) Injection of p53-MOspl targeting p53 mRNA rescued the mutant phenotype. Embryos 70 h post-injection were used for whole-mount in situ hybridization using a trypsin probe. (mu) Mutant; (wt) wild type. (C) Semiquantitative RT-PCR examination of expression of various p53 response genes in defhi429 mutant (mu) and the wild-type control (wt) at 3, 4, and 5 dpf, respectively. Homozygous defhi429 mutants (confirmed by lacking expression of def) had elevated levels of Δ113p53, cyclin G1, p21WAF1/CIP1, and mdm2 but unchanged levels of p53, reprimo, and bax when compared with the wild type (wt). (D) At 3 dpf, whole-mount in situ hybridization using cyclin G1, mdm2, and p21WAF1/CIP-1 probes showed that the expression of these genes was significantly and specifically increased in the digestive organs. (in) Intestine; (lv) liver; (p) pancreas.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
baxa WT standard conditions Protruding-mouth to Day 5 unspecified RTPCR
utp25hi429Tg standard conditions Protruding-mouth to Day 5 unspecified RTPCR
ccng1 WT standard conditions Protruding-mouth gut ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Protruding-mouth to Day 5 unspecified RTPCR
utp25hi429Tg standard conditions Protruding-mouth gut ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Protruding-mouth to Day 5 unspecified RTPCR
cdkn1a WT standard conditions Protruding-mouth intestine ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Protruding-mouth pharynx ISH
Protruding-mouth to Day 5 unspecified RTPCR
utp25hi429Tg standard conditions Protruding-mouth intestine ISH
Protruding-mouth liver ISH
Protruding-mouth pancreas ISH
Protruding-mouth pharynx ISH
Protruding-mouth to Day 5 unspecified RTPCR
eef1a1l1 WT standard conditions Protruding-mouth to Day 5 unspecified RTPCR
utp25hi429Tg standard conditions Protruding-mouth to Day 5 unspecified RTPCR
mdm2 WT standard conditions Protruding-mouth unspecified ISH
Protruding-mouth to Day 5 unspecified RTPCR
utp25hi429Tg standard conditions Protruding-mouth unspecified ISH
Protruding-mouth to Day 5 unspecified RTPCR
prss1 WT standard conditions Pec-fin to Protruding-mouth exocrine pancreas ISH
utp25hi429Tg + MO3-tp53 standard conditions Pec-fin to Protruding-mouth exocrine pancreas ISH
tp53 WT standard conditions Protruding-mouth to Day 5 unspecified RTPCR
utp25hi429Tg standard conditions Protruding-mouth to Day 5 unspecified RTPCR
utp25 WT standard conditions Protruding-mouth to Day 5 unspecified RTPCR
utp25hi429Tg standard conditions Protruding-mouth to Day 5 unspecified RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Genes & Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.
In addition, ZFIN would like to thank the authors for kindly granting us permission to reproduce their figures.
Full text @ Genes & Dev.