Fig. 1

The def^hi429 mutant exhibits hypoplastic digestive organs. (A,B) At 5.5 dpf, the def^hi429 mutant had an unabsorbed york sac and exhibited smaller liver and swimbladder and much thinner intestine as seen by dissection microscopes. The liver is outlined with green, the gut with red, the swimbladder with purple, and the york sac with yellow. (mu) Mutant; (wt) wild-type. (C,D) At 5 dpf, Phenol Red injection revealed that the def^hi429 mutant had a smaller gallbladder (g) and underexpanded anterior intestine (in). The gut from anterior to posterior (left to right) is outlined with a black line. (E,F) At 5 dpf, alcian blue staining showed that the branchial arches 2-7 in the def^hi429 mutant (mu) were deformed. Branchial arches 1-7 in wild type are indicated in E. (G-J) Histochemical staining of alkaline phosphatase showed that pronephric ducts (G,H) at 3 dpf and blood vessels (I,J) at 4 dpf in wild type (wt) and the def^hi429 mutant (mu) were indistinguishable. (bv) Blood vessels; (pd) pronephric ducts.