Connors et al., 1999 - The role of tolloid/mini fin in dorsoventral pattern formation of the zebrafish embryo. Development (Cambridge, England)   126(14):3119-3130

Fig. 3

Morphological defects visible in live mini fin mutant embryos. (A,B) 1-somite-stage mfn mutant (B) displays an oblong shape relative to wild type (A). 5- somite-stage wild-type (C) and mfn (D) mutant embryos. Arrows denote the tail bud length and the asterisk marks a protrusion of the tail bud seen in the mutant (D). 24 hpf wild type (E) and tails of mfn mutants exhibiting (F) a partial loss of tail fin (between the arrows) that extends to the dorsal side, (G) partial loss of the ventral fin with a bifurcation in the tail, (H) near complete absence of ventral fin and a kink in the tail. This occasionally observed kink is associated with a disruption in the notochord, which is also observed in the more strongly dorsalized mutant lost-a-fin (Solnica-Krezel et al., 1996, our unpublished observations). (I,J) mfn homozygous adult fish. Homozygous adult exhibiting a near wild-type appearance (I). Note that a pigmented stripe in the tail is disrupted in this mutant, a trait characteristic of mfn homozygous fish. Other adult mutants may display a partial or full (J) loss of their tail fin. All are lateral views. (A-D) dorsal to the right, (E-J) dorsal to the top. AF, anal fin; TF, tail fin; DF, dorsal fin.

Fig. 5

Absence or reduction of three ventral tail cell types in mfn mutants. msxD expression in 8-somite-stage wild-type (A) and mfn mutant (B) embryos. msxB expression in 14-somite-stage wild-type (C) and mfn mutant (D) embryos. msxB is also expressed in other more anterior cell types. dlx3 expression in 24 hpf wildtype (E) and mutant (F-H) embryos. flk1 expression in 24 hpf wild-type (I) and mutant (J,K) embryos. Note reduced flk1 in mfn (arrows). myoD expression in 32 hpf wild-type (L) and mutant (M,N) embryos. Arrowheads in B and D denote reduced/absent gene expression in the mutant. (F-H) Ventral dlx3 expression is absent between the arrows. All are lateral views. (A-D) Dorsal is to the right, anterior to the top. (E-N) Dorsal is to the top, anterior to the left.

Fig. 6

tld expression pattern and altered tld, chd and bmp4 expression in mutant embryos. eve1 expression in bud stage wild-type (A) and mfn mutant (B) embryos. bmp4 expression in 1-somite-stage wild-type (C) and mfn mutant (D) embryos. tld expression in wild-type embryos at 55% epiboly (E), 70% epiboly (F,G), 85% epiboly (H), bud-stage (I) and 20-somite-stage (J) wild-type embryos. Arrowhead in J points to tld expression in the presumptive vasculature. Inset in J is a higher magnification showing the posterior trunk of a slightly older embryo with tld expression marking the prospective artery and vein (arrows). tld expression at 5 somites in wild-type (K), chordino (L) and swirl (M) mutant embryos. Insets in K-L are higher magnification caudal views of tld expression, anterior to the top. Reduced tld expression (arrowhead) in a swirl mutant. Double in situ of chd (magenta) and bmp4 (blue) expression in a bud-stage wild-type embryo (N). Double in situ of chd (magenta) and tld (blue) expression in bud-stage wild-type (O) and mfn mutant (P) embryos. (P) Expanded chd expression (arrows) in a mfn mutant. (A-D, G-M) Lateral views, dorsal to the right. (E) Dorsal view, slightly tilted downward. (F) Animal pole view. (N-P) Vegetal pole view, anterior/dorsal to the top.

ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.