Mutation of jam3b reduces npas4l expression in the PLPM. (A) Expression of gata1a, scl, etv2, fli1a and npas4l in the PLPM of WT or jam3bsa37 embryos at 14 hpf. (B) Relative expression levels of npas4l and fli1a in isolated fli1a:GFP (+) cells from WT or jam3bsa37 embryos at 15 hpf. Error bars represent s.d. (n=3 each). (C) Expression of spi1b, scl and etv2 in the ALPM of WT or jam3bsa37 embryos at 14 hpf. (D) Expression of npas4l at 11 hpf (left) and fli1a at 12 hpf (right) in the PLPM of WT or jam3bsa37 embryos. Numbers in bottom right of panels indicate the number of embryos showing the displayed expression pattern over the total number of analyzed embryos. Scale bars: 100 μm.