ZFIN ID: ZDB-FIG-200401-7
Kobayashi et al., 2019 - Dual role of Jam3b in early hematopoietic and vascular development. Development (Cambridge, England)   147(1) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 10-13 somites

Fig. 2 Mutation of jam3b reduces npas4l expression in the PLPM. (A) Expression of gata1a, scl, etv2, fli1a and npas4l in the PLPM of WT or jam3bsa37 embryos at 14 hpf. (B) Relative expression levels of npas4l and fli1a in isolated fli1a:GFP (+) cells from WT or jam3bsa37 embryos at 15 hpf. Error bars represent s.d. (n=3 each). (C) Expression of spi1b, scl and etv2 in the ALPM of WT or jam3bsa37 embryos at 14 hpf. (D) Expression of npas4l at 11 hpf (left) and fli1a at 12 hpf (right) in the PLPM of WT or jam3bsa37 embryos. Numbers in bottom right of panels indicate the number of embryos showing the displayed expression pattern over the total number of analyzed embryos. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etsrp WT standard conditions 10-13 somites anterior lateral plate mesoderm ISH
10-13 somites posterior lateral plate mesoderm ISH
jam3bsa37/sa37 standard conditions 10-13 somites anterior lateral plate mesoderm ISH
10-13 somites posterior lateral plate mesoderm ISH
fli1 WT standard conditions 5-9 somites posterior lateral plate mesoderm ISH
10-13 somites posterior lateral plate mesoderm ISH
jam3bsa37/sa37 standard conditions 5-9 somites posterior lateral plate mesoderm ISH
10-13 somites anterior lateral plate mesoderm ISH
10-13 somites posterior lateral plate mesoderm ISH
gata1a WT standard conditions 10-13 somites posterior lateral plate mesoderm ISH
jam3bsa37/sa37 standard conditions 10-13 somites posterior lateral plate mesoderm ISH
npas4l WT standard conditions 1-4 somites mesoderm ISH
10-13 somites posterior lateral plate mesoderm ISH
jam3bsa37/sa37 standard conditions 1-4 somites mesoderm ISH
10-13 somites posterior lateral plate mesoderm ISH
spi1b WT standard conditions 10-13 somites anterior lateral plate mesoderm ISH
jam3bsa37/sa37 standard conditions 10-13 somites posterior lateral plate mesoderm ISH
tal1 WT standard conditions 10-13 somites anterior lateral plate mesoderm ISH
10-13 somites posterior lateral plate mesoderm ISH
jam3bsa37/sa37 standard conditions 10-13 somites anterior lateral plate mesoderm ISH
10-13 somites posterior lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jam3bsa37/sa37 standard conditions 10-13 somites anterior lateral plate mesoderm etsrp expression decreased amount, abnormal
10-13 somites anterior lateral plate mesoderm fli1 expression decreased amount, abnormal
10-13 somites anterior lateral plate mesoderm tal1 expression decreased amount, abnormal
10-13 somites posterior lateral plate mesoderm fli1 expression decreased amount, abnormal
10-13 somites posterior lateral plate mesoderm tal1 expression decreased amount, abnormal
10-13 somites posterior lateral plate mesoderm spi1b expression decreased amount, abnormal
10-13 somites posterior lateral plate mesoderm etsrp expression decreased amount, abnormal
10-13 somites posterior lateral plate mesoderm npas4l expression decreased amount, abnormal
10-13 somites posterior lateral plate mesoderm gata1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development