ZFIN ID: ZDB-FIG-200401-11
Kobayashi et al., 2019 - Dual role of Jam3b in early hematopoietic and vascular development. Development (Cambridge, England)   147(1) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 10-13 somites to Prim-5

Fig. 6 Jam3b regulates the integrin signaling pathway to form HSCs. (A) Fluorescent image of a fli1a:Gal4; UAS:mCherry-itgb1aDN embryo. (B) Expression of runx1 in the DA of mCherry-itgb1aDN (−), or mCherry-itgb1aDN (+) embryos uninjected or injected with ca-faka mRNA. (C) Expression of lrrc15 in the vascular cord of mCherry-itgb1aDN (−) or mCherry-itgb1aDN (+) embryos. (D) Expression of runx1 in the DA of mCherry-itgb1aDN (+) embryos uninjected or injected with lrrc15 mRNA. (E) Time-lapse images of fli1a:GFP in WT or jam3bsa37 embryos. Dotted lines indicate the boundary of eleventh and twelfth somite at each time point. White arrowheads indicate a subset of fli1a:GFP (+) cells that show a rounded morphology. Data are representative of four embryos for each genotype. (F) Expression of lrrc15 in the vascular cord of WT, or jam3bsa37 embryos uninjected or injected with drl mRNA, ca-faka mRNA, or both drl and ca-faka mRNA. (G) Expression of runx1 in the DA of WT, or jam3bsa37 embryos uninjected or injected with ca-faka mRNA or both drl and ca-faka mRNA. (H) Expression of lrrc15 in the vascular cord of WT embryos treated with DMSO or wortmannin. (I) Expression of runx1 in the DA of WT embryos treated with wortmannin, either uninjected or injected with lrrc15 mRNA. Percent distribution of runx1 expression in I is shown in Fig. S8C. Black arrowheads indicate the expression domain of each gene. Numbers in bottom right of panels indicate the number of embryos showing the displayed expression pattern over the total number of analyzed embryos. Scale bars: 50 μm (E); 100 μm (A-D,F-H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP jam3bsa37; y1Tg standard conditions 10-13 somites to 14-19 somites endothelial cell IFL
y1Tg standard conditions 10-13 somites to 14-19 somites endothelial cell IFL
lrrc15 WT standard conditions 14-19 somites vascular cord ISH
20-25 somites vascular cord ISH
WT chemical treatment: wortmannin 20-25 somites vascular cord ISH
jam3bsa37/sa37 standard conditions 14-19 somites Not Detected vascular cord ISH
20-25 somites Not Detected vascular cord ISH
ko112Tg standard conditions 20-25 somites Not Detected vascular cord ISH
mCherry ubs4Tg ; ko112Tg standard conditions Prim-5 endothelial cell IFL
runx1 WT standard conditions Prim-5 dorsal aorta ISH
jam3bsa37/sa37 standard conditions Prim-5 dorsal aorta ISH
ko112Tg standard conditions Prim-5 dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: wortmannin 20-25 somites vascular cord lrrc15 expression decreased amount, abnormal
jam3bsa37/sa37 standard conditions 14-19 somites vascular cord lrrc15 expression absent, abnormal
20-25 somites vascular cord lrrc15 expression absent, abnormal
Prim-5 dorsal aorta runx1 expression decreased distribution, abnormal
jam3bsa37; y1Tg standard conditions 10-13 somites - 14-19 somites endothelial cell circular, abnormal
10-13 somites - 14-19 somites endothelial cell migration decreased rate, abnormal
ko112Tg standard conditions 20-25 somites vascular cord lrrc15 expression absent, abnormal
Prim-5 dorsal aorta runx1 expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development