ZFIN ID: ZDB-FIG-200320-5
Chou et al., 2018 - Ribose-5-Phosphate Isomerase A Overexpression Promotes Liver Cancer Development in Transgenic Zebrafish via Activation of ERK and β-catenin Pathways. Carcinogenesis   40(3):461-473 Full text @ Carcinogenesis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 5 The expression of β-catenin and downstream target genes was upregulated in RPIA-transgenic fish aged 7–11 months. (A) Representative images of β-catenin IHC staining from 5 to 11 months of control (mC), Tg1- and Tg2-RPIA-transgenic fish. A weaker β-catenin signal was detected in transgenic, control tissues (mC) relative to tissues from the RPIA-transgenic fish, and staining in the control fish revealed expression in the area surrounding the nucleus. In the 9 and 11 months of Tg (fabp10a: RPIA) fish lines, the amount of β-catenin in nucleus was upregulated. All of the magnifications were ×400. Scale bar: 50 μm. (B) Statistical analysis of β-catenin immunostaining in 5- to 11-month-old Tg1- and Tg2-RPIA-transgenic fish compared with control. Statistical analysis was performed by unpaired two-tailed t-tests. Asterisks (*) represent the level of significance. *P < 0.05 was considered statistically significant. **P < 0.01; ***P < 0.001. N = 10 each stage. (C) Immunofluorescence staining of β-catenin in Tg1- and Tg2-RPIA-transgenic zebrafish compared with control (mC). The images showed that β-catenin (green) lost the expression for cell junction and co-localized with DAPI (blue) within nucleus in RPIA-transgenic zebrafish liver. All of the magnifications were ×400. Scale bar: 40 μm. (D–F) QPCR was used to measure the induction of the β-catenin target genes ccnd1 (D), cdkn2a/b (E), and mycb (F) in two independent lines of Tg (fabp10a: RPIA), which compared with control fish (mC) aged 3, 5, 7, 9 and 11 months. A significant increase in the expression levels of β-catenin target genes was found in 9- to 11-month-old Tg1 and 7- to 9-month-old Tg2 transgenic fish as compared with the transgenic control fish (mCherry). Statistical analysis was performed by unpaired two-tailed t-tests. Asterisks (*) represent the level of significance. *P < 0.05 was considered statistically significant. **P < 0.01; ***P < 0.001. N = 10 each stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnd1 nn1009aTg standard conditions Adult liver RTPCR
nn1009bTg standard conditions Adult liver RTPCR
cdkn2a/b nn1009aTg standard conditions Adult liver RTPCR
nn1009bTg standard conditions Adult liver RTPCR
mycb nn1009aTg standard conditions Adult liver RTPCR
nn1009bTg standard conditions Adult liver RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab13-ctnnb IHC nn1000Tg standard conditions Adult liver
IHC nn1009aTg standard conditions Adult liver
IHC nn1009bTg standard conditions Adult liver
Phenotype Details
Fish Conditions Stage Phenotype
nn1009aTg standard conditions Adult liver ab13-ctnnb labeling increased amount, abnormal
Adult liver mycb expression increased amount, abnormal
Adult liver cdkn2a/b expression increased amount, abnormal
Adult liver ccnd1 expression increased amount, abnormal
nn1009bTg standard conditions Adult liver ab13-ctnnb labeling increased amount, abnormal
Adult liver cdkn2a/b expression increased amount, abnormal
Adult liver ccnd1 expression increased amount, abnormal
Adult liver mycb expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Carcinogenesis for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Carcinogenesis