ZFIN ID: ZDB-FIG-200320-1
Chou et al., 2018 - Ribose-5-Phosphate Isomerase A Overexpression Promotes Liver Cancer Development in Transgenic Zebrafish via Activation of ERK and β-catenin Pathways. Carcinogenesis   40(3):461-473 Full text @ Carcinogenesis
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 1 Steatosis and fibrosis develop in RPIA-transgenic zebrafish. (A) Representative pictures of Oil Red O staining results from liver tissues of 3- and 5-month-old control (fabp10a: EGFP-mCherry, mC) and two Tg (fabp10a: RPIA) lines (Tg1 and Tg2) magnified ×400. Scale bar: 50 μm. (B) Statistical analysis of the Oil Red O staining results for the 3- and 5-month-old RPIA Tg 1 (orange diamond) and Tg2 (red triangle) compared with the control fish (mC, blue). Ten fish from each group were analyzed. (C) Representative image of Sirius Red staining results of the 3- to 7- month-old control (mC) and two RPIA-transgenic fish (Tg1 and Tg2). Control fish exhibited no staining while 7-month-old Tg1 fish and 5-month-old Tg2 fish displayed positive signs of fibrosis, which are shown in red. All of the magnifications were ×400. Scale bar: 50 μm (D) Statistical analysis of the Sirius Red staining results for the 3-, 5- and 7-month-old RPIA Tg 1 (orange diamond) and Tg2 (red triangle) compared with the control fish (mC, blue). (E) The ratio of NADPH/NADP+ was elevated in 5-month-old RPIA-transgenic zebrafish. P < 0.05 was considered to be statistically significant; *: 0.01 < P ≤ 0.05.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nn1009aTg standard conditions Adult liver fatty, abnormal
Adult liver fibrotic, abnormal
nn1009bTg standard conditions Adult liver fatty, abnormal
Adult liver fibrotic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Carcinogenesis for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Carcinogenesis