ZFIN ID: ZDB-FIG-200320-4
Chou et al., 2018 - Ribose-5-Phosphate Isomerase A Overexpression Promotes Liver Cancer Development in Transgenic Zebrafish via Activation of ERK and β-catenin Pathways. Carcinogenesis   40(3):461-473 Full text @ Carcinogenesis
Anatomical Terms:
Stage: Adult
Observed In:
Stage: Adult

Fig. 4 pERK expression is elevated in RPIA-transgenic fish. (A) Representative images of pERK IHC results for the control (mC), and two RPIA-transgenic fish (Tg1 and Tg2) aged 7–11 months. The control fish displayed only a weak pERK signal. The 7- to 11-month-old Tg1 and Tg2 fish exhibited significant stronger staining. All the magnifications were ×400. Scale bar: 50 μm. (B) Statistical analysis of the IHC results for pERK expression of two independent Tg (fabp10a: RPIA)—Tg1 and Tg2 compared with control (mC) fish. Statistical analysis was performed by unpaired two-tailed t-tests. Asterisks (*) represent the level of significance. *P < 0.05 was considered statistically significant. **P < 0.01; ***P < 0.001. (C) Immunofluorescence staining of pERK in two independent RPIA-transgenic zebrafish (Tg1 and Tg2) compared with control (mC). The images showed that pERK (green) and DAPI (blue) immunofluorescent signals co-localized within nucleus in RPIA-transgenic zebrafish liver. All of the magnifications were ×400. Scale bar: 40 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab9-mapk IHC nn1000Tg standard conditions Adult liver
IHC nn1009aTg standard conditions Adult liver nucleus
IHC nn1009bTg standard conditions Adult liver nucleus
Phenotype Details
Fish Conditions Stage Phenotype
nn1009aTg standard conditions Adult liver nucleus ab9-mapk labeling increased amount, abnormal
nn1009bTg standard conditions Adult liver nucleus ab9-mapk labeling increased amount, abnormal
ZFIN wishes to thank the journal Carcinogenesis for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Carcinogenesis