ZFIN ID: ZDB-FIG-200320-3
Chou et al., 2018 - Ribose-5-Phosphate Isomerase A Overexpression Promotes Liver Cancer Development in Transgenic Zebrafish via Activation of ERK and β-catenin Pathways. Carcinogenesis   40(3):461-473 Full text @ Carcinogenesis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 3 RPIA-transgenic fish increase expression of cell cycle/proliferation markers and PCNA nuclear staining. (A–C) QPCR was used to measure the relative mRNA fold induction of the cell cycle/proliferation markers ccne1, cdk1 and cdk2 in two independent lines of RPIA-transgenic fish (Tg1 and Tg2) compared with the control fish (mC) at 3, 5, 7, 9 and 11 months of age. (D) Representative images of PCNA IHC result for the control (mC) and Tg1 and Tg2 fish aged 5–11 months. All of the magnifications were ×400. Scale bar: 50 μm. (E) Statistical analysis of the PCNA IHC staining results from Tg1 and Tg2 compared with control, as determined by the IRS scoring system. Statistical analysis was performed by two-tailed Student’s t-tests. The symbol ‘*’ represents significance between RPIA-transgenic fish and GFP-mCherry control. P < 0.05 was considered to be statistically significant; *: 0.01 < P ≤ 0.05; **: 0.001 < P ≤ 0.01; ***: P ≤ 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccne1 nn1000Tg standard conditions Adult liver RTPCR
nn1009aTg standard conditions Adult liver RTPCR
nn1009bTg standard conditions Adult liver RTPCR
cdk1 nn1000Tg standard conditions Adult liver RTPCR
nn1009aTg standard conditions Adult liver RTPCR
nn1009bTg standard conditions Adult liver RTPCR
cdk2 nn1000Tg standard conditions Adult liver RTPCR
nn1009aTg standard conditions Adult liver RTPCR
nn1009bTg standard conditions Adult liver RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-pcna IHC nn1000Tg standard conditions Adult liver
IHC nn1009aTg standard conditions Adult liver
IHC nn1009bTg standard conditions Adult liver
Phenotype Details
Fish Conditions Stage Phenotype
nn1009aTg standard conditions Adult liver cdk1 expression increased amount, abnormal
Adult liver cdk2 expression increased amount, abnormal
Adult liver ccne1 expression increased amount, abnormal
Adult liver ab2-pcna labeling increased amount, abnormal
nn1009bTg standard conditions Adult liver cdk2 expression increased amount, abnormal
Adult liver cdk1 expression increased amount, abnormal
Adult liver ccne1 expression increased amount, abnormal
Adult liver ab2-pcna labeling increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Carcinogenesis for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Carcinogenesis