ZFIN ID: ZDB-FIG-191003-3
Xue et al., 2019 - A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis. Cell Reports   27:1567-1578.e5 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. S6

The specific role of integrin pathway for HSPC expansion, related to figure 5.

A. The Venn diagram showing the overlapped receptors from the comprehensive analysis of three sequencing methods.

B. The Venn diagram showing the overlapped ligands from the comprehensive analysis of three sequencing methods.

C. WISH showing the expression of ctgfa and itgb2 at 2 dpf, 3 dpf and 4 dpf in zebrafish CHT region. 

D. The validation of ctgfa and itgb2 morpholino efficiency by fusion GFP plasmid injection.

E. WISH showing the expression of runx1 in control embryos, itgb2 morphants and ctgfa morphants at 30 hpf.

F. Confocal imaging of the transgenic line (CD41:GFP/gata1;dsRed) showing hematopoietic cells in the CHT region in control embryos, itgb2 morphants and ctgfa morphants at 3.5 dpf. 

G. The quantification data of CD41:GFP cells number in (F). Data are shown as means +- SEM. n=4, ns (no signifigant) P>0.05, *P<0,05.

H. WISH the expression of showing cmyb, gatal, coro1a and mfap4 in control embryos, ctgfa morphants and itgb2 morphants at 4.5 dpf.

I. qPCR results showing the expression of cmyb, pu. 1 and gatal in control embryos, ctgfa morphants and itgb2 morphants at 4 dpf. Data are shown as means +- SEM. n=3, **P<0.01, ***P<0.001.

J. WISH data showing the expression of ctgfa in GFP+ and GFP- embryos at 3.5 dpf after heat shocking transgenic (hsp701:ccn2a-2A-EGFP) embryos.

K. WISH results displaying the expression of cmyb at 4dpf in control embryos, ctgfa morphants, ctgfa MO injected hsp701:ccn2a-2A-EGFP+ embryos.

L. The identification of itgb2 mutants by sequancing analysis of genomic DNA with seven base pairs deletion.

M. The illustration showing the normal protein structure in wild-type embryos and the truncated protein structure in itgb2 mutants.

N. WISH data showing the expression of cmyb in sibling and itgb2 mutants 4dpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
coro1a TU + MO1-itgb2 control Day 4 caudal hematopoietic tissue ISH
TU + MO3-ccn2a control Day 4 caudal hematopoietic tissue ISH
gata1a TU + MO1-itgb2 control Day 4 caudal hematopoietic tissue ISH
Day 4 whole organism RTPCR
TU + MO3-ccn2a control Day 4 caudal hematopoietic tissue ISH
Day 4 whole organism RTPCR
mfap4 TU + MO1-itgb2 control Day 4 caudal hematopoietic tissue ISH
TU + MO3-ccn2a control Day 4 caudal hematopoietic tissue ISH
myb TU + MO1-itgb2 control Day 4 caudal hematopoietic tissue ISH
Day 4 whole organism RTPCR
TU + MO3-ccn2a control Day 4 caudal hematopoietic tissue ISH
Day 4 whole organism RTPCR
itgb2ioz105/ioz105 control Day 4 caudal hematopoietic tissue ISH
pd97Tg heat shock Day 4 caudal hematopoietic tissue ISH
pd97Tg + MO3-ccn2a heat shock Day 4 caudal hematopoietic tissue ISH
runx1 TU + MO1-itgb2 control Day 4 ventral wall of dorsal aorta ISH
TU + MO3-ccn2a control Prim-15 ventral wall of dorsal aorta ISH
spi1b TU + MO1-itgb2 control Day 4 whole organism RTPCR
TU + MO3-ccn2a control Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-itgb2 control Day 4 caudal hematopoietic tissue coro1a expression increased amount, abnormal
Day 4 caudal hematopoietic tissue myb expression increased amount, abnormal
Day 4 caudal hematopoietic tissue mfap4 expression increased amount, abnormal
Day 4 caudal hematopoietic tissue gata1a expression increased amount, abnormal
Day 4 whole organism myb expression increased amount, abnormal
Day 4 whole organism spi1b expression increased amount, abnormal
Day 4 whole organism gata1a expression increased amount, abnormal
TU + MO3-ccn2a control Day 4 caudal hematopoietic tissue coro1a expression increased amount, abnormal
Day 4 caudal hematopoietic tissue myb expression increased amount, abnormal
Day 4 caudal hematopoietic tissue mfap4 expression increased amount, abnormal
Day 4 caudal hematopoietic tissue gata1a expression increased amount, abnormal
Day 4 whole organism spi1b expression increased amount, abnormal
Day 4 whole organism gata1a expression increased amount, abnormal
Day 4 whole organism myb expression increased amount, abnormal
itgb2ioz105/ioz105 control Day 4 caudal hematopoietic tissue myb expression increased amount, abnormal
pd97Tg heat shock Day 4 caudal hematopoietic tissue myb expression decreased amount, abnormal
pd97Tg + MO3-ccn2a heat shock Day 4 caudal hematopoietic tissue myb expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.