ZFIN ID: ZDB-FIG-190802-2
Xue et al., 2019 - A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis. Cell Reports   27:1567-1578.e5 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 5

The Crosstalk between CHT Niche and HSPCs

(A) SOM showing the distinct gene regulatory structure among NCs, ECs, and HSPCs at 52 hpf and 3 and 4 dpf.

(B) Secretomics analysis showing the ligand-receptor regulatory relationship between HSPCs and CHT niche at 52 hpf.

(C) The secretomics analysis for scRNA-seq data revealing the interaction pairs in distinct cell types, receptor-expressing C4 (proliferating HSPC) and ligand-expressing clusters.

(D) The distribution of ligands and receptors in ECs (kdrl+ cells), HSPCs (CD41+cells), and NCs (kdrl-CD41- cells) at 52 hpf and 3 dpf based on bulk RNA-seqanalysis.

(E) Double FISH showing the co-expression of ctgfa with fli1a, and itgb2 with cmyb at 2 dpf. Scale bar, 50 μm.

(F) The confocal imaging of the transgenic line (kdrl:mCherry/runx1:en-GFP) showing the number of runx1+ cells in control, ctgfa morphants, and itgb2morphants at 4 dpf. Scale bar, 50 μm.

(G) The quantification of confocal imaging displayed in (F) are shown as means ± SEM. p < 0.05, n = 8. The HSPCs were counted in about the eight-somite region of the CHT in control embryos and morphants.

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccn2a TU control Long-pec caudal hematopoietic tissue vasculature ISH
fli1 TU control Long-pec caudal hematopoietic tissue vasculature ISH
GFP ioz1Tg; pku6Tg control Day 4 caudal hematopoietic tissue IFL
ioz1Tg; pku6Tg + MO1-itgb2 control Day 4 caudal hematopoietic tissue IFL
ioz1Tg; pku6Tg + MO3-ccn2a control Day 4 caudal hematopoietic tissue IFL
itgb2 TU control Long-pec caudal hematopoietic tissue ISH
Long-pec caudal hematopoietic tissue hematopoietic multipotent progenitor cell ISH
mCherry ioz1Tg; pku6Tg control Day 4 vasculature IFL
ioz1Tg; pku6Tg + MO1-itgb2 control Day 4 vasculature IFL
ioz1Tg; pku6Tg + MO3-ccn2a control Day 4 vasculature IFL
myb TU control Long-pec caudal hematopoietic tissue hematopoietic multipotent progenitor cell ISH
Long-pec hematopoietic multipotent progenitor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ioz1Tg; pku6Tg + MO1-itgb2 control Day 4 caudal hematopoietic tissue GFP expression increased amount, abnormal
Day 4 caudal hematopoietic tissue GFP expression increased distribution, abnormal
Day 4 caudal hematopoietic tissue hematopoietic cell increased amount, abnormal
ioz1Tg; pku6Tg + MO3-ccn2a control Day 4 caudal hematopoietic tissue GFP expression increased amount, abnormal
Day 4 caudal hematopoietic tissue GFP expression increased distribution, abnormal
Day 4 caudal hematopoietic tissue hematopoietic cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.