ZFIN Figure: Park et al., 2019, Fig. 2

ZFIN ID: ZDB-FIG-190827-26

ZFIN ID: ZDB-FIG-190827-26

Park et al., 2019 - Low dose of chronic ethanol exposure in adult zebrafish induces hepatic steatosis and injury. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 117:109179 Full text @ Biomed. Pharmacother.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	cebpa dffa dgat2 fasn ldlra lpl srebf1 srebf2
Fish:	WT
Condition:	chemical treatment by environment: ethanol
Anatomical Term:	liver
Stage:	Adult

PHENOTYPE:

Fish:	WT
Condition:	chemical treatment by environment: ethanol
Observed In:	liver liver triglyceride
Stage:	Adult

Fig. 2

Chronic ethanol treatment induced steatosis in the liver of adult zebrafish. (A) The relative mRNA expression of genes associated with lipid metabolism include lipogenic transcription factors, lipid synthesis, lipid uptake, and fatty acid transporting into the mitochondria. (B) Oil red O staining the section of liver in untreated control (left) and 0.2% ethanol treated wild type zebrafish. Scale bar = 50 μm. (C) The quantification of triglycerides in the liver of control and ethanol treated zebrafish. Error bars indicate standard deviation of the mean. * p < 0.05, ** p < 0.005.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
cebpa	WT	chemical treatment by environment: ethanol	Adult	liver	RTPCR
dffa	WT	chemical treatment by environment: ethanol	Adult	liver	RTPCR
dgat2	WT	chemical treatment by environment: ethanol	Adult	liver	RTPCR
fasn	WT	chemical treatment by environment: ethanol	Adult	liver	RTPCR
ldlra	WT	chemical treatment by environment: ethanol	Adult	liver	RTPCR
lpl	WT	chemical treatment by environment: ethanol	Adult	liver	RTPCR
srebf1	WT	chemical treatment by environment: ethanol	Adult	liver	RTPCR
srebf2	WT	chemical treatment by environment: ethanol	Adult	liver	RTPCR

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT	chemical treatment by environment: ethanol	Adult	liver fatty, abnormal
		Adult	liver srebf2 expression increased amount, abnormal
		Adult	liver lpl expression increased amount, abnormal
		Adult	liver srebf1 expression increased amount, abnormal
		Adult	liver dffa expression increased amount, abnormal
		Adult	liver cebpa expression increased amount, abnormal
		Adult	liver ldlra expression increased amount, abnormal
		Adult	liver fasn expression increased amount, abnormal
		Adult	liver dgat2 expression increased amount, abnormal
		Adult	liver triglyceride increased amount, abnormal

Acknowledgments:
ZFIN wishes to thank the journal Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biomed. Pharmacother.