ZFIN ID: ZDB-FIG-180713-10
Zhang et al., 2018 - The DEAD box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish. Development (Cambridge, England)   145(8) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 3

Loss of ddx39ab results in defective skeletal muscle differentiation in zebrafish. (A) Immunostaining demonstrates the organization of myofilaments in wild-type and ddx39ab mutant embryos at 24 hpf. Anti-MHC antibody (MF20) labels thick (myosin) filaments and F-actin was visualized by phalloidin staining. Scale bars: 20 μm. (B) RNA in situ hybridization for myogenic regulatory gene expression in wild-type and ddx39ab mutant zebrafish embryos at 32 hpf. (C,D) RNA in situ hybridization and qPCR analysis for myocyte structural gene expression in wild-type and ddx39ab mutant zebrafish embryos at 32 hpf. (B,C) Lateral views with anterior to the left. (D) Transverse section with dorsal side to top. At least 15 (A) or 20 (B-D) embryos of each genotype were analyzed and representative samples are shown. For qPCR results, data are mean±s.e.m. n.s, not significant. **P<0.01, ***P<0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
casq1a WT standard conditions Prim-15 somite ISH
ddx39abnju203Gt/nju203Gt standard conditions Prim-15 somite ISH
Prim-15 whole organism RTPCR
myf5 WT standard conditions Prim-15 somite ISH
ddx39abnju203Gt/nju203Gt standard conditions Prim-15 somite ISH
myhz2 WT standard conditions Prim-15 somite ISH
ddx39abnju203Gt/nju203Gt standard conditions Prim-15 somite ISH
Prim-15 whole organism RTPCR
myod1 WT standard conditions Prim-15 somite ISH
ddx39abnju203Gt/nju203Gt standard conditions Prim-15 somite ISH
myog WT standard conditions Prim-15 somite ISH
ddx39abnju203Gt/nju203Gt standard conditions Prim-15 somite ISH
Prim-15 whole organism RTPCR
slc25a4 WT standard conditions Prim-15 somite ISH
ddx39abnju203Gt/nju203Gt standard conditions Prim-15 somite ISH
Prim-15 whole organism RTPCR
smyhc1 WT standard conditions Prim-15 somite ISH
ddx39abnju203Gt/nju203Gt standard conditions Prim-15 somite ISH
Prim-15 whole organism RTPCR
tnnt2d WT standard conditions Prim-15 somite ISH
ddx39abnju203Gt/nju203Gt standard conditions Prim-15 somite ISH
Prim-15 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Prim-5 muscle myosin filament
IHC ddx39abnju203Gt/nju203Gt standard conditions Prim-5 muscle myosin filament
Phenotype Details
Fish Conditions Stage Phenotype
ddx39abnju203Gt/nju203Gt standard conditions Prim-5 muscle myofilament disorganized, abnormal
Prim-15 somite casq1a expression decreased amount, abnormal
Prim-15 somite myhz2 expression decreased amount, abnormal
Prim-15 somite myog expression decreased amount, abnormal
Prim-15 somite slc25a4 expression decreased amount, abnormal
Prim-15 somite smyhc1 expression decreased amount, abnormal
Prim-15 somite tnnt2d expression decreased amount, abnormal
Prim-15 whole organism casq1a expression decreased amount, abnormal
Prim-15 whole organism myhz2 expression decreased amount, abnormal
Prim-15 whole organism myog expression increased amount, abnormal
Prim-15 whole organism slc25a4 expression decreased amount, abnormal
Prim-15 whole organism smyhc1 expression decreased amount, abnormal
Prim-15 whole organism tnnt2d expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development