ZFIN ID: ZDB-FIG-180208-14
Donat et al., 2018 - Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis. eLIFE   7:e28939 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 4

Notch activity within endocardial cushion cells is affected in ccm mutants.

(A, B) Whole-mount in situ hybridization of notch1b cardiac expression at 48 hpf. (A) notch1b expression is restricted to endocardial cells of the atrioventricular canal (AVC) in wild-type (WT), while notch1b is strongly expressed throughout all ventricular cells in krit1ty219c mutants (B). (C, D) Projections of single confocal z-section images of endocardial cells marked by Tg(TP1:VenusPEST)s940 expression (yellow) and Alcam staining (magenta) at 54 hpf. (C) In WT, Notch activity is highest in endocardial cells of the AVC and outflow regions. (C') Single confocal plane section of the AVC (white box in C) reveals that some endocardial cells close to the ventricle lack Notch activity (asterisks). (D) In ccm2m201 mutants, the domain of high Notch activity is expanded to most ventricular endocardial cells. (D') Single confocal plane section of the AVC region (box in D, arrow) shows high Notch expression in all endocardial cells of the AVC region. A: atrium, V: ventricle, Scale bars are 50 μm (C, D), and 10 µm (C', D').

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
alcama zn-8 ccm2m201/m201 ; s940Tg standard conditions Long-pec endocardium IHC
zn-8 s940Tg standard conditions Long-pec endocardium IHC
notch1b WT standard conditions Long-pec Not Detected atrial endocardium ISH
Long-pec atrioventricular canal endocardium ISH
Long-pec Not Detected ventricular endocardium ISH
krit1ty219c/ty219c standard conditions Long-pec atrial endocardium ISH
Long-pec ventricular endocardium ISH
Venus ccm2m201/m201 ; s940Tg standard conditions Long-pec atrioventricular canal endocardium IHC
Long-pec ventricular endocardium IHC
s940Tg standard conditions Long-pec atrioventricular canal endocardium IHC
Long-pec ventricular endocardium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ccm2m201/m201 ; s940Tg standard conditions Long-pec atrioventricular canal endocardium Venus expression increased distribution, abnormal
Long-pec atrioventricular canal endocardium Notch signaling involved in heart development increased occurrence, abnormal
Long-pec atrioventricular valve morphogenesis decreased process quality, abnormal
Long-pec ventricular endocardium Venus expression increased distribution, abnormal
Long-pec ventricular endocardium Notch signaling involved in heart development increased occurrence, abnormal
krit1ty219c/ty219c standard conditions Long-pec atrial endocardium notch1b expression mislocalised, abnormal
krit1ty219c/ty219c standard conditions Long-pec atrioventricular valve morphogenesis decreased process quality, abnormal
krit1ty219c/ty219c standard conditions Long-pec ventricular endocardium notch1b expression mislocalised, abnormal
krit1ty219c/ty219c standard conditions Long-pec ventricular endocardium Notch signaling involved in heart development increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife