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ZFIN ID: ZDB-FIG-180208-8
Donat et al., 2018 - Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis. eLIFE   7:e28939 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 2

Desensitization of endocardial cells to blood-flow by heg1, krit1, or ccm2 overexpression.

(A) Correlation of high heg1 and low klf2a mRNA expression levels at 24 and 48 hpf as determined by RT-qPCR. (B–D) Projections of confocal z-stack images of 54 hpf hearts expressing the flow-responsive marker Tg(TP1:VenusPEST)s940 in heat-shocked wild-type (WT) (B), heat-shock- (hs) induced krit1 overexpression (C), and hs-induced ccm2 overexpression (D). (E) Different ratios of corrected total tissue fluorescence (CTTF) of atrial versus ventricular (+atrioventricular canal region (AVC), arrowhead) expression of the flow-responsive marker Tg(TP1:VenusPEST)s940 between 54 hpf WT and krit1-overexpressing hearts (n = 37) and WT and ccm2-overexpressing hearts (n = 39). krit1- or ccm2-overexpression results in more equal chamber expression of flow-responsive marker Tg(TP1:VenusPEST)s940 expression (depicted is a WT heart corresponding to the krit1-overexpression experiment). (F) Fold change of relative fluorescence (CTTF) in the AVC of Tg(TP1:VenusPEST)s940 in krit1- and ccm2- overexpressing hearts versus the corresponding controls. Scale bars are 50 µm. Mean values ± SEM are shown. Unpaired t-test was used to compare each condition with the WT (*p<0.05, **p<0.01, ***p<0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Venus md6Tg ; s940Tg heat shock Long-pec atrial endocardium IFL
Long-pec atrioventricular canal endocardium IFL
Long-pec ventricular endocardium IFL
s940Tg heat shock Long-pec atrioventricular canal endocardium IFL
Long-pec ventricular endocardium IFL
s940Tg ; md12Tg heat shock Long-pec atrial endocardium IFL
Long-pec atrioventricular canal endocardium IFL
Long-pec ventricular endocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
md6Tg ; s940Tg heat shock Long-pec atrial endocardium Venus expression mislocalised, abnormal
Long-pec atrioventricular canal endocardium Venus expression decreased amount, abnormal
Long-pec ventricular endocardium Venus expression decreased amount, abnormal
s940Tg ; md12Tg heat shock Long-pec atrial endocardium Venus expression mislocalised, abnormal
Long-pec atrioventricular canal endocardium Venus expression decreased amount, abnormal
Long-pec ventricular endocardium Venus expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife