ZFIN ID: ZDB-FIG-180118-10
Reporter knock-in into the otx2 locus does not affect endogenous otx2 expression. otx2:venus and otx2:tRFP fish were crossed and sorted into either single (Venus+ or tRFP+) or double positive (Venus+ and tRFP+) embryos at 24 hpf. Images were taken from live embryos anesthetized in MS-222. Panel (A) fluorescent images of the single- and double-positive embryos show no morphological abnormalities. Images are maximum intensity projections covering 50 μm tissue with a Z-interval of 2 μm. Scale bar: 50 μm. Images were taken from live embryos anesthetized in MS-222. Panel (B) Double in situ hybridization for otx2 (blue) and the MHB marker pax2a (red) show no differences in gene expression pattern or morphology of the MHB region between the single- and double-positive groups. Mb, midbrain; Hb, hindbrain. Scale bar 50 μm.
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