ZFIN ID: ZDB-FIG-180118-10
Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development. Frontiers in Neuroanatomy   11:52 Full text @ Front. Neuroanat.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 5

Reporter knock-in into the otx2 locus does not affect endogenous otx2 expression. otx2:venus and otx2:tRFP fish were crossed and sorted into either single (Venus+ or tRFP+) or double positive (Venus+ and tRFP+) embryos at 24 hpf. Images were taken from live embryos anesthetized in MS-222. Panel (A) fluorescent images of the single- and double-positive embryos show no morphological abnormalities. Images are maximum intensity projections covering 50 μm tissue with a Z-interval of 2 μm. Scale bar: 50 μm. Images were taken from live embryos anesthetized in MS-222. Panel (B) Double in situ hybridization for otx2 (blue) and the MHB marker pax2a (red) show no differences in gene expression pattern or morphology of the MHB region between the single- and double-positive groups. Mb, midbrain; Hb, hindbrain. Scale bar 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
otx2b tud40Tg/+(AB) control Prim-5 midbrain ISH
Prim-5 midbrain hindbrain boundary ISH
tud40Tg/+ ; tud41Tg/+(AB) control Prim-5 midbrain ISH
Prim-5 midbrain hindbrain boundary ISH
tud41Tg/+(AB) control Prim-5 midbrain ISH
Prim-5 midbrain hindbrain boundary ISH
pax2a tud40Tg/+(AB) control Prim-5 midbrain hindbrain boundary ISH
tud40Tg/+ ; tud41Tg/+(AB) control Prim-5 midbrain hindbrain boundary ISH
tud41Tg/+(AB) control Prim-5 midbrain hindbrain boundary ISH
RFP tud40Tg/+ ; tud41Tg/+(AB) control Prim-5 midbrain IFL
Prim-5 midbrain hindbrain boundary IFL
tud41Tg/+(AB) control Prim-5 midbrain IFL
Prim-5 midbrain hindbrain boundary IFL
Venus tud40Tg/+(AB) control Prim-5 midbrain IFL
Prim-5 midbrain hindbrain boundary IFL
tud40Tg/+ ; tud41Tg/+(AB) control Prim-5 midbrain IFL
Prim-5 midbrain hindbrain boundary IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Neuroanatomy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neuroanat.