ZFIN ID: ZDB-FIG-180118-13
Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development. Frontiers in Neuroanatomy   11:52 Full text @ Front. Neuroanat.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 9

otx2:venus expression in the adult zebrafish midbrain. (A) Schematic representation of the adult zebrafish brain. The position of the cross section of the midbrain with optic tectum, hypothalamus, and pituitary shown in panel (B) is indicated (orientation indicators A->P: anterior to posterior; D->V: dorsal to ventral). (B) Cross section of the midbrain labeled with otx2:venus (green), the pan neuronal marker HuC/D (red) and the nuclear counterstain DAPI (blue). (C) Higher magnification image of the region indicated by white box in panel (B) showing that numerous neurons express otx2:venus. (D–F) Higher magnification images of the regions indicated by white boxes in (C). otx2:venus is co-expressed in HuC/D expressing neurons (yellow arrowheads) in the various tectal sub layers that is characteristic of a teleost midbrain. However, a sub-population of HuC/D positive neurons was negative for otx2:venus (white arrowheads). (D) Type III horizontal neurons that belong to the stratum opticum. (E) Projection neurons that mostly belong to the stratum griseum et album superficiale and centrale regions. (F) Prominent unipolar type XIV interneurons located in the periventricular gray zone of optic tectum. Anatomical descriptions are based on the zebrafish brain atlas (Wullimann et al., 1996), tectal sub layers and neuronal types were interpreted based on Meek (1981) and Meek and Schellart (1978). Scale bars (B): 100 μm; (C): 20 μm; (D–F): 5 μm. (B,C: maximum intensity projection; D–F: single Z-Plane).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Venus tud40Tg(AB) control Adult hypothalamus neuron IHC
Adult optic tectum neuron IHC
Adult optic tectum ventricular zone IHC
Adult periventricular grey zone neuron IHC
Adult periventricular grey zone unipolar neuron IHC
Adult stratum fibrosum et griseum superficiale neuron IHC
Adult stratum fibrosum et griseum superficiale neuron projection IHC
Adult stratum opticum neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC tud40Tg(AB) control Adult hypothalamus neuron
IHC Adult optic tectum neuron
IHC Adult periventricular grey zone neuron
IHC Adult periventricular grey zone unipolar neuron
IHC Adult stratum fibrosum et griseum superficiale neuron
IHC Adult stratum opticum neuron
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Neuroanatomy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neuroanat.