ZFIN ID: ZDB-FIG-180118-8
Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development. Frontiers in Neuroanatomy   11:52 Full text @ Front. Neuroanat.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 2

Targeted knock-in of Venus fluorescent protein into the otx2 locus. Expression of Venus fluorescent protein at 24 hpf. Images were taken from live embryos anesthetized in MS-222. (A) Left panel shows dorsal view with the anterior of the embryo facing upwards, right panel is a merged image of the fluorescent and transmitted light channels. Venus is expressed in the retina and midbrain, sharply abutting the MHB (dotted line). (B) Lateral view of an embryo expressing Venus. Left panel shows fluorescent channel and right panel shows a merged image of the fluorescent and transmitted light channels. All images are maximum intensity projections covering 50 μm tissue with a Z-interval of 2 μm. Mb, midbrain; Hb, hindbrain. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Venus tud40Tg(AB) control Prim-5 midbrain IFL
Prim-5 midbrain hindbrain boundary IFL
Prim-5 optic cup IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Neuroanatomy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neuroanat.