ZFIN ID: ZDB-FIG-180118-14
Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development. Frontiers in Neuroanatomy   11:52 Full text @ Front. Neuroanat.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 10

otx2:venus expression in radial glia located at the ventricular zone of the adult zebrafish midbrain. (A) Schematic representation of the adult zebrafish brain. The position of the cross section of the midbrain with optic tectum, hypothalamus, and pituitary shown in panel (B) is indicated (orientation indicators A->P: anterior to posterior; D->V: dorsal to ventral). (B) Cross section of the midbrain labeled with otx2:venus (green), the radial glial marker S100 (red) and the nuclear counterstain DAPI (blue). (C) Higher magnification of the region from midbrain tectum indicated by white boxes in (B). (D) Higher magnification of an inset from panel (C) showing radial glial cells at the ventricular zone of the midbrain tectum labeled by S100 co-expressing otx2:venus. Anatomical descriptions are based on the zebrafish brain atlas (Wullimann et al., 1996). Scale bars (B): 100 μm; (C): 25 μm; (D): 2 (or) 5 μm. (B,C: maximum intensity projection; D: single Z-plane).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Venus tud40Tg(AB) control Adult midbrain IHC
Adult optic tectum glial cell IHC
Adult optic tectum ventricular zone IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Neuroanatomy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neuroanat.