ZFIN ID: ZDB-FIG-180118-12
Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development. Frontiers in Neuroanatomy   11:52 Full text @ Front. Neuroanat.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 7

Targeted knock-in of fluorescent reporters into the pax2a locus. Expression of Venus or tRFP driven by the pax2a locus at 24 hpf. Images were taken from live embryos anesthetized in MS-222. (A) Left panel shows dorsal view of Venus expression in the MHB and otic vesicle (OV); right panel shows merged image of fluorescent and transmitted light channels. (B) Left panel shows dorsal view of tRFP expression in the MHB and otic vesicle. (C) Left panel: lateral view of an embryo expressing tRFP in the optic stalk (OS), MHB, OV, and hindbrain neurons (Hbn). Right panel: merged image of fluorescent and transmitted light channels. (D) pax2a:venus and pax2a:tRFP fish were crossed and resulting progeny was sorted into either single (Venus+ or tRFP+) or double positive (Venus+ and tRFP+) embryos at 24 hpf. Compared to single-positive siblings, double positive embryos show no morphological abnormalities. All images are maximum intensity projections covering 50 μm tissue with a Z-interval of 2 μm. Scale bar 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
RFP tud42Tg/+ ; tud43Tg/+(AB) control Prim-5 midbrain hindbrain boundary IFL
Prim-5 otic vesicle IFL
tud43Tg(AB) control Prim-5 hindbrain neuron IFL
Prim-5 midbrain hindbrain boundary IFL
Prim-5 optic stalk IFL
Prim-5 otic vesicle IFL
tud43Tg/+(AB) control Prim-5 midbrain hindbrain boundary IFL
Prim-5 otic vesicle IFL
Venus tud42Tg(AB) control Prim-5 midbrain hindbrain boundary IFL
Prim-5 otic vesicle IFL
tud42Tg/+(AB) control Prim-5 midbrain hindbrain boundary IFL
Prim-5 otic vesicle IFL
tud42Tg/+ ; tud43Tg/+(AB) control Prim-5 midbrain hindbrain boundary IFL
Prim-5 otic vesicle IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Neuroanatomy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neuroanat.