ZFIN ID: ZDB-FIG-180118-16
Kesavan et al., 2017 - CRISPR/Cas9-Mediated Zebrafish Knock-in as a Novel Strategy to Study Midbrain-Hindbrain Boundary Development. Frontiers in Neuroanatomy   11:52 Full text @ Front. Neuroanat.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 12

pax2a:venus expression in the adult zebrafish midbrain. (A) Scheme indicating a plane of coronal section of the adult zebrafish mid brain with optic tectum, hypothalamus and the pituitary corresponding to the cross section in “B” (orientation indicators A->P: anterior to posterior; D->V: Dorsal to Ventral). (B) Cross section of the midbrain immunostained against venus (green) and DAPI (blue). (C) in situ hybridization against pax2a in the midbrain similar to the coronal planes in “A,B” showing similar expression pattern as that of venus in the knock in reporter line. (D,F,H) Higher magnification of a midbrain indicated by white boxes in “B” showing neuronal nuclei expressing venus in regions, valvula cerebelli (red dotted line area) and dorsal tegmental nucleus (yellow dotted line area) “D,” neighboring (left side) to the lateral longitudinal fascicle “F” and the rostral tegmental nucleus “H.” (E,G,H) Higher magnification of a region from the midbrain tectum indicated by black boxes in “C” showing in situ expression pattern for pax2a showing highly similar pattern to their counter parts expressing pax2a:venus. Anatomical descriptions were based on (zebrafish brain atlas; Wullimann et al., 1996). Scale bars (B,C): 100 μm; (D,E,G,I): 20 μm; (F,H): 10 μm. (B: max intensity projection; D,F,H: single Z-Plane; C,E,G,I: DIC image).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax2a AB control Adult midbrain ISH
Adult optic tectum ISH
Adult rostral tegmental nucleus ISH
Adult valvula cerebelli neuron ISH
Venus tud42Tg(AB) control Adult midbrain IFL
Adult optic tectum IFL
Adult rostral tegmental nucleus IFL
Adult valvula cerebelli neuron IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Neuroanatomy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neuroanat.