ZFIN ID: ZDB-FIG-171120-37
Gao et al., 2016 - Depletion of Myostatin b Promotes Somatic Growth and Lipid Metabolism in Zebrafish. Frontiers in endocrinology   7:88 Full text @ Front Endocrinol (Lausanne)
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 3

General characterization of the muscle tissues of mstnb-deficient zebrafish. (A) Paraffin section and H&E staining of mstnb-deficient zebrafish (A3, A4) and wild-type control fish (A1, A2). Lower magnification images are shown in A1 and A3; higher magnification of the indicated regions of A1 and A3 are shown in A2 and A4. (B) The distribution of muscle numbers are according to theirs sizes. The Y-axis indicates the numbers of muscle fibers at certain cross areas (μm2) of <100, 100–250, 250–450, and >450. The dotted rectangles in A1 and A3 define the areas for analysis. (C) The total fiber number of mstnb−/− and mstnb+/+ zebrafish sections. (D) The diagram shows the total area of muscle tissue in the body cross-section. (E) Western blot analysis of slow myofiber-specific protein S58 from muscle tissue of mstnb+/+ (left panel) and mstnb−/− (right panel). GAPDH protein was used as an internal control. (F) The western blot analysis was quantified by gray value analysis using Image J software. (G) Transcriptional expression levels of myogenesis genes. * indicate significant differences at (P < 0.05).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myf5 WT standard conditions Adult muscle RTPCR
mstnbzf1027/zf1027 standard conditions Adult muscle RTPCR
myf6 WT standard conditions Adult muscle RTPCR
mstnbzf1027/zf1027 standard conditions Adult muscle RTPCR
myod1 WT standard conditions Adult muscle RTPCR
mstnbzf1027/zf1027 standard conditions Adult muscle RTPCR
myog WT standard conditions Adult muscle RTPCR
mstnbzf1027/zf1027 standard conditions Adult muscle RTPCR
pax3a WT standard conditions Adult muscle RTPCR
mstnbzf1027/zf1027 standard conditions Adult muscle RTPCR
pax7a WT standard conditions Adult muscle RTPCR
mstnbzf1027/zf1027 standard conditions Adult muscle RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-S58 WB WT standard conditions Adult muscle
WB mstnbzf1027/zf1027 standard conditions Adult muscle
Phenotype Details
Fish Conditions Stage Phenotype
mstnbzf1027/zf1027 standard conditions Adult muscle ab-s58 labeling decreased amount, abnormal
Adult muscle hyperplastic, abnormal
Adult muscle myog expression increased amount, abnormal
Adult muscle myod1 expression increased amount, abnormal
Adult muscle myf5 expression increased amount, abnormal
Adult muscle cell hyperplastic, abnormal
Adult skeletal muscle cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in endocrinology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Endocrinol (Lausanne)