ZFIN Image: Gao et al., 2016, Fig. 3

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Figure Caption

Fig. 3

General characterization of the muscle tissues of mstnb-deficient zebrafish. (A) Paraffin section and H&E staining of mstnb-deficient zebrafish (A3, A4) and wild-type control fish (A1, A2). Lower magnification images are shown in A1 and A3; higher magnification of the indicated regions of A1 and A3 are shown in A2 and A4. (B) The distribution of muscle numbers are according to theirs sizes. The Y-axis indicates the numbers of muscle fibers at certain cross areas (μm²) of <100, 100–250, 250–450, and >450. The dotted rectangles in A1 and A3 define the areas for analysis. (C) The total fiber number of mstnb−/− and mstnb+/+ zebrafish sections. (D) The diagram shows the total area of muscle tissue in the body cross-section. (E) Western blot analysis of slow myofiber-specific protein S58 from muscle tissue of mstnb+/+ (left panel) and mstnb−/− (right panel). GAPDH protein was used as an internal control. (F) The western blot analysis was quantified by gray value analysis using Image J software. (G) Transcriptional expression levels of myogenesis genes. * indicate significant differences at (P < 0.05).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)