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ZFIN ID: ZDB-FIG-170914-43
Nguyen-Chi et al., 2017 - TNF signaling and macrophages govern fin regeneration in zebrafish larvae. Cell Death & Disease   8:e2979 Full text @ Cell Death Dis.
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. S6

Effect of tnfa morpholino on caudal fin regeneration (a) Fin images are representative transmitted light images of Ctrl morphants and tnfa morphants at 3dpA. (b) Corresponding regenerated fin length (mean ±s.e.m. from three independent experiments, ***P<0.001). (c) Schematic representation of the parabiosis experiment using tnfa MO or WT (CTRL) and Tg(mpeg1:GAL4/UAS:NTR-mCherry). Parabiotic larvae were treated with MTZ at 48 hpf and the caudal fin of Tg(mpeg1:GAL4/UAS:NTR-mCherry) was amputated at 3 dpf. (d) Representative fin images of mCherry fluorescence merged with transmitted channel at 6 hpA show NTRmCherry+ macrophages that are mainly depleted. White arrowheads show residual fluorescence in cells or cell fragments. (e) Fin images are representative transmitted light images at 3 dpA in tnfa morphants and CTRL. (f) Corresponding quantification of the regenerated fin length in indicated conditions (Nlarvae=8-10 mean ± s.e.m., *P<0.05). In (a, d and e) dotted lines outline the fin, dashed arrows indicate the position of the initial transection. Scale bars = 100 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO5-tnfa amputation: caudal fin Protruding-mouth fin regeneration decreased efficacy, abnormal
Protruding-mouth regenerating fin decreased length, abnormal
ZFIN wishes to thank the journal Cell Death & Disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Dis.