ZFIN ID: ZDB-FIG-170914-37
Nguyen-Chi et al., 2017 - TNF signaling and macrophages govern fin regeneration in zebrafish larvae. Cell Death & Disease   8:e2979 Full text @ Cell Death Dis.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 5 to Day 6

Fig. 4

Depletion of late-recruited macrophages affects regeneration by impairing mesenchymal cell behavior but not cell death. (a and b) Representative images show cell death (green) in intact and amputated fin at (a) 48hpA and (b) 24hpA using confocal microscopy. (a) Cell death was detected using Acridine Orange staining in larvae in which all macrophages were depleted with L-clodronate treatment 24h before amputation (L-clodronate 1) or not (L-PBS). (b) Cell death was detected using Acridine Orange staining in larvae in which late macrophages were depleted with L-clodronate treatment at 6hpA (L-clodronate 2) or not (L-PBS). Dotted lines outline the fin, asterisks show autofluorescence of the pigments and arrowheads show dead cells. Scale bar=100μm. (c,d) Cell death counts in indicated conditions (Nlarvae=7–18 per group from two independent experiments, mean values±S.E.M., *P<0.05). (e) Tg(rcn3:gal4/UAS:Ds-Red) larvae were amputated at 3dpf and injected with either L-PBS or L-clodronate at 6hpA. Fin images are representative confocal maximum projections of Ds-Red (magenta) fluorescence in mesenchymal cells of the fins at 72hpA. Arrowheads show round mesenchymal cells at the wound of late macrophage-depleted larvae. Scale bar=100 μm

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry pd1023Tg ; pd1112Tg chemical treatment by injection: clondronate(2-), chemical ablation: macrophage, amputation: caudal fin Day 6 notochord IFL
Day 6 regenerating fin mesenchymal cell IFL
pd1023Tg ; pd1112Tg amputation: caudal fin Day 6 notochord IFL
Day 6 regenerating fin mesenchymal cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by injection: clondronate(2-), chemical ablation: macrophage, amputation: caudal fin Day 5 regenerating fin cell death increased occurrence, abnormal
pd1023Tg ; pd1112Tg chemical treatment by injection: clondronate(2-), chemical ablation: macrophage, amputation: caudal fin Day 6 regenerating fin mesenchymal cell morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Death & Disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Dis.