ZFIN ID: ZDB-FIG-170914-35
Nguyen-Chi et al., 2017 - TNF signaling and macrophages govern fin regeneration in zebrafish larvae. Cell Death & Disease   8:e2979 Full text @ Cell Death Dis.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 4 to Day 6

Fig. 2

Macrophages are required for fin regeneration and blastemal cell proliferation in a stage-dependent manner. (a) Schedule of macrophage (Mφ) depletion using L-clodronate injections. Macrophages were ablated using early L-clodronate injections (L-clodronate 1; no macrophages at the wound) or using injection of L-clodronate at later stages (L-clodronate 2; no more recruited M2-like macrophages from 24hpA). (b and e) Consequences of macrophage depletion on caudal fin regeneration. (b) To deplete all macrophages from the early stage of regeneration, Tg(mpeg1:mCherry-F) were injected with L-clodronate (or L-clo) or L-PBS (control) at 48hpf and fin were transected at 72hpf (L-clodronate 1). (e) To deplete late-recruited macrophages, Tg(mpeg1:mCherry-F) were injected with L-clodronate or L-PBS at 6hpA (L-clodronate 2). Fin images are representative overlays of mCherry fluorescence and transmitted light acquisitions at 3dpA. Scale bar=100μm. Dotted lines outline the fin and dashed arrows, the position of the initial amputation. (c and f) Corresponding quantification of the regenerated fin length at 3dpA after L-clodronate 1 treatment (c) and L-clodronate 2 treatment (f) in indicated conditions (mean±S.E.M., **P<0.01 and ***P<0.001). (dg) Blastema cell proliferation at 24hpA after L-clodronate 1 (d) and 2 (g) treatments in indicated conditions. Mitotic cells were detected using an anti-phosphorylated histone H3 (PH3) antibody (Nlarvae=10–15, average value of cut/uncut ratio±S.E.M, *P<0.05)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry ump2Tg chemical treatment by injection: clondronate(2-), chemical ablation: macrophage, amputation: caudal fin Day 6 regenerating fin macrophage IFL
ump2Tg amputation: caudal fin Day 6 regenerating fin macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ump2Tg chemical treatment by injection: clondronate(2-), chemical ablation: macrophage, amputation: caudal fin Day 4 blastema cell population proliferation decreased occurrence, abnormal
Day 6 fin regeneration decreased efficacy, abnormal
Day 6 macrophage decreased amount, abnormal
Day 6 regenerating fin decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Death & Disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Dis.