FIGURE

Fig. 1

ID
ZDB-FIG-170914-34
Publication
Nguyen-Chi et al., 2017 - TNF signaling and macrophages govern fin regeneration in zebrafish larvae
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

Tnfa+ and tnfa macrophages are differentially recruited during caudal fin regeneration in zebrafish larvae. (a) Diagram showing the amputation plan (red arrow) and the counting region (pink box) in the 3 dpf larvae. (b) Caudal fin of Tg(mpeg1:mCherry-F/ tnfa:eGFP-F) were amputated at 3dpf as shown in A and mCherry-F and GFP-F expressions were analyzed by confocal microscopy at 6hpA in either intact (Ctrl) or amputated fins. Fin images are representative maximum projections of single fluorescence channels (mCherry-F, GFP-F) and overlay of fluorescences with transmitted light images (Merge). Arrowheads show mCherry-F+GFP-F+ cells and asterisk GFP-F+ cell, dotted lines outline the fin. Scale bar=20μm. (c) Number of tnfa+ and tnfa macrophages recruited to the wound region at indicated time points post amputation over 3 days. The number of larvae (N) used at each time point is indicated on the top of the graph. **P< 0.01 and ***P<0.001 indicated time point versus 0hpA. Below the graph, a sketch of the different phases of recruitment of macrophage subsets: the M1-like macrophages accumulate during the early phase of regeneration (phase 1), whereas M2-like macrophages peaked at a later stage of the process (phase 2)

Expression Data
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.