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ZFIN ID: ZDB-FIG-170914-36
Nguyen-Chi et al., 2017 - TNF signaling and macrophages govern fin regeneration in zebrafish larvae. Cell Death & Disease   8:e2979 Full text @ Cell Death Dis.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 6

Fig. 3

Genetic depletion of macrophages at different stages confirms the requirement of macrophages during fin regeneration. (a) Schedule of macrophage (Mφ) depletion using Tg(mpeg1:GAL4/UAS:NTR-mCherry) larvae and Metronidazol (MTZ) treatment. (be) Tg(mpeg1:GAL4/UAS:NTR-mCherry) larvae were treated with MTZ (NTR+ MTZ+) to deplete macrophages. DMSO treatments on the same line (NTR+ MTZ) or MTZ treatments on WT siblings (NTR MTZ+) were used as controls. (b) Treatments were performed from 48hpf and fins were amputated at 3dpf to deplete macrophages during the whole regeneration process (MTZ 1). (d) Treatments were performed from 6hpA to deplete late macrophages (MTZ 2). Fin images are representative overlays of mCherry-F fluorescence (red) with transmitted light acquisitions at 3dpA. Scale bar=100μm. Dotted lines outline the fin; dashed arrows indicate the position of the initial amputation. (c and e) Corresponding quantification of the regenerated fin length at 3dpA in MTZ 1 (c) and MTZ 2 treatments (e) in indicated conditions (mean±S.E.M. from three independent experiments, ***P<0.001 and *P<0.05)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry c264Tg ; gl24Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole, amputation: caudal fin Day 6 regenerating fin macrophage IFL
c264Tg ; gl24Tg amputation: caudal fin Day 6 regenerating fin macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
c264Tg ; gl24Tg chemical ablation: macrophage, chemical treatment by environment: metronidazole, amputation: caudal fin Day 6 fin regeneration decreased efficacy, abnormal
Day 6 macrophage decreased amount, abnormal
Day 6 regenerating fin decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Death & Disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Dis.