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Fig. S2

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ZDB-FIG-170410-21

Publication

Crespo et al., 2014 - The PAR complex controls the spatiotemporal dynamics of F-actin and the MTOC in directionally migrating leukocytes

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Fig. S2

F-actin anteroposterior polarity in live myeloid cells correlates with cell speed, related to Fig. 2. (A) Top panel, one cycle of front-rear F-actin waves is visualized in wounded TG(FmpoP::memYFP) larvae co-expressing transiently RFP-Lifeact and PKC-ζ-WT in myeloid cells. Shown are frames taken at the indicated times from representative movies of migrating leukocytes in the wounded tailfin. The white arrows indicate direction of migration. Scale bar = 10 μm. Bottom panel, time profile of cell’s speed. Black dots correspond to the indicated time points. (B) Fluorescence signal distribution of F-actin in “back” and “front” regions of the cell at each time point ti is plotted against cellular speed (determined using the positions at ti-1 and ti+1 as described in the Materials and Methods). For each range of speed, data represents means ± s.e.m. (521 counts, 16 leukocytes from 3 larvae; Back: Spearman r = 0.61, Front: Spearman r = - 0.57, **** p (two-tailed) < 0.0001). (C) Fluorescence signal distribution in (B) is normalized to memYFP intensity levels and plotted against cellular speed. For each range of speed, data represents means ± s.e.m. (Back: Spearman r = 0.69, Front: Spearman r = -0.67, **** p (two41 tailed) < 0.0001).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.