FIGURE

Fig. 8

ID

ZDB-FIG-170410-19

Publication

Crespo et al., 2014 - The PAR complex controls the spatiotemporal dynamics of F-actin and the MTOC in directionally migrating leukocytes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

PKC-ζ regulates RhoA activity in leukocytes migrating to wounds in vivo. (A) Frames from representative movies of migrating myeloid cells in wounded tailfins are shown. The white arrows indicate the direction of migration. Cell outlines are shown as white lines. Scale bars: 10 µm. See also supplementary material Movie 12. RhoA activity was visualized in TG(FmpoP::mCherry) larvae transiently expressing cytosolic RhoA-FRET biosensor together with PKC-ζ-WT or PKC-ζ-KW in myeloid cells. Ratiometric images of YFP∶CFP emission for each cell are shown. (B) Average activation level of RhoA (mean emission ratio of YFP∶CFP for the entire cell during migration) in wound-activated leukocytes. Data are expressed as the mean±s.e.m. of all analyzed cells (PKC-ζ-WT, 25 leukocytes in 15 larvae; PKC-ζ-KW, 15 leukocytes in 5 larvae); **P<0.01 (two-tailed unpaired Student's t-test). (C) A model illustrating the mechanism by which the PAR complex controls wound-directed leukocyte migration in vivo. The PAR complex coordinately controls Rho-dependent F-actin dynamics and MTOC perinuclear mobility to support the persistent migration of leukocytes to wounds.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.