ZFIN ID: ZDB-FIG-170208-31
Hu et al., 2016 - Liver-Enriched Gene 1, a Glycosylated Secretory Protein, Binds to FGFR and Mediates an Anti-stress Pathway to Protect Liver Development in Zebrafish. PLoS Genetics   12:e1005881 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Prim-15

Fig. 3

Loss-of-function of Leg1 blocks the liver bud formation.

(A) Loss of maternal zygotic Leg1a affects the liver development. Embryos were treated with or without UV25 at 24hpf, WISH was performed to assess the development of the liver (fabp10a), exocrine (trypsin) and endocrine (insulin) pancreas and intestine (fabp2a) in the maternal-zygotic leg1azju1(mu) embryos at 3.5 dpf. At least three independent WISH was performed, each time with 24–31 embryos for each sample, and representative embryos were shown. (B) WISH using prox1 and hhex to examine liver bud formation at 30 hpf in embryos treated with or without UV25 treatment at 24 hpf. Representative pictures in each group are presented. The number of embryos exhibiting the phenotype over total embryos examined are shown in the bracket. Red arrow: liver bud, blue arrow: pancreatic bud. (C and D) Images (C) and statistical analysis (D) of PH3 immunostaining to compare cell proliferation of hepatocytes in WT and maternal-zygotic leg1azju1 embryos (mu) after UV25 treatment. BHMT is an enzyme highly expressed in the liver and was used to mark out the hepatocytes. DAPI was used to stain the nuclei. Red arrows indicate PH3 positive cells in the liver. PH3 positive cells in the neural tube in WT and the mutant were also recorded in parallel. *, p<0.05. in, intestinal tube, lv, liver, nt, neural tube.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bhmt Ab1-bhmt leg1.1zju6/zju6 control Prim-15 liver IHC
Ab1-bhmt leg1.1zju6/zju6 radiation Prim-15 liver IHC
fabp2 WT control Protruding-mouth intestine ISH
WT radiation Protruding-mouth intestine ISH
leg1.1zju6/zju6 control Protruding-mouth intestine ISH
leg1.1zju6/zju6 radiation Protruding-mouth intestine ISH
fabp10a WT control Protruding-mouth liver ISH
WT radiation Protruding-mouth liver ISH
leg1.1zju6/zju6 control Protruding-mouth liver ISH
leg1.1zju6/zju6 radiation Protruding-mouth liver ISH
hhex WT control Prim-15 liver ISH
Prim-15 pancreatic bud ISH
WT radiation Prim-15 liver ISH
Prim-15 pancreatic bud ISH
leg1.1zju6/zju6 control Prim-15 liver ISH
Prim-15 pancreatic bud ISH
leg1.1zju6/zju6 radiation Prim-15 Not Detected liver ISH
Prim-15 pancreatic bud ISH
ins WT control Protruding-mouth endocrine pancreas ISH
WT radiation Protruding-mouth endocrine pancreas ISH
leg1.1zju6/zju6 control Protruding-mouth endocrine pancreas ISH
leg1.1zju6/zju6 radiation Protruding-mouth endocrine pancreas ISH
prox1a WT control Prim-15 liver ISH
Prim-15 pancreatic bud ISH
WT radiation Prim-15 liver ISH
Prim-15 pancreatic bud ISH
leg1.1zju6/zju6 control Prim-15 liver ISH
Prim-15 pancreatic bud ISH
leg1.1zju6/zju6 radiation Prim-15 Not Detected liver ISH
Prim-15 pancreatic bud ISH
prss1 WT control Protruding-mouth exocrine pancreas ISH
WT radiation Protruding-mouth exocrine pancreas ISH
leg1.1zju6/zju6 control Protruding-mouth exocrine pancreas ISH
leg1.1zju6/zju6 radiation Protruding-mouth exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
leg1.1zju6/zju6 radiation Prim-15 liver cell population proliferation process quality, abnormal
Prim-15 liver development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.