The compensatory mechanism is activated in leg1a mutant.
(A) WISH analysis of the liver sizes in WT and maternal-zygotic leg1azju1(mu) at 3.5 and 10 dpf using the fabp10a probe. Left panel: histogram showing the percentage of normal, intermediate and small liver in the WT and leg1a mutant embryos at 3.5 and 10 dpf. The number of embryos (n) was shown on the top. Right panel: representative image of 10-dpf embryos after WISH. (B and C) leg1b expression in the zygotic mutant embryos at 4 dpf (B) and maternal-zygotic mutant embryos at 1, 3, 5 and 7dpf(C). (D) Comparison of the liver to body ratio between WT and leg1azju1mutant in the adult fish. Each dot represents the ratio for an individual adult fish. Error bar stands for the standard error. (E) Representative images of the WT and leg1azju1mutant adult fish. (F) Survival rate of the WT and leg1azju1mutant adult fish aged at 9, 14 and 18 months. The starting number of the larvae fish for each counting (SN) are shown on the top.