ZFIN ID: ZDB-FIG-170208-33
Hu et al., 2016 - Liver-Enriched Gene 1, a Glycosylated Secretory Protein, Binds to FGFR and Mediates an Anti-stress Pathway to Protect Liver Development in Zebrafish. PLoS Genetics   12:e1005881 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Adult

Fig. 8

The compensatory mechanism is activated in leg1a mutant.

(A) WISH analysis of the liver sizes in WT and maternal-zygotic leg1azju1(mu) at 3.5 and 10 dpf using the fabp10a probe. Left panel: histogram showing the percentage of normal, intermediate and small liver in the WT and leg1a mutant embryos at 3.5 and 10 dpf. The number of embryos (n) was shown on the top. Right panel: representative image of 10-dpf embryos after WISH. (B and C) leg1b expression in the zygotic mutant embryos at 4 dpf (B) and maternal-zygotic mutant embryos at 1, 3, 5 and 7dpf(C). (D) Comparison of the liver to body ratio between WT and leg1azju1mutant in the adult fish. Each dot represents the ratio for an individual adult fish. Error bar stands for the standard error. (E) Representative images of the WT and leg1azju1mutant adult fish. (F) Survival rate of the WT and leg1azju1mutant adult fish aged at 9, 14 and 18 months. The starting number of the larvae fish for each counting (SN) are shown on the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fabp10a leg1.1zju6/zju6 standard conditions Days 7-13 liver ISH
leg1.2 leg1.1zju6/zju6 standard conditions Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
leg1.1zju6/zju6 standard conditions Day 4 whole organism RTPCR
leg1.1zju6/zju6 standard conditions Day 5 whole organism RTPCR
Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
leg1.1zju6/zju6 standard conditions Protruding-mouth whole organism leg1.2 expression increased amount, abnormal
leg1.1zju6/zju6 standard conditions Day 4 whole organism leg1.2 expression increased amount, abnormal
leg1.1zju6/zju6 standard conditions Day 5 whole organism leg1.2 expression increased amount, abnormal
Days 7-13 whole organism leg1.2 expression increased amount, abnormal
leg1.1zju6/zju6 standard conditions Adult axis decreased length, abnormal
Adult liver size, normal
Adult whole organism decreased life span, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.