ZFIN Figure: Ahuja et al., 2016, Fig. 5

ZFIN ID: ZDB-FIG-160427-6

ZFIN ID: ZDB-FIG-160427-6

Ahuja et al., 2016 - Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development. Developmental Biology 412(1):71-82 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	alcama mCherry
Fish:	s896Tg id4^bns18/bns18; s896Tg
Anatomical Terms:	atrioventricular canal endocardium atrioventricular canal endocardium cell
Stage:	Long-pec

PHENOTYPE:

Fish:	id4^bns18/bns18; s896Tg
Observed In:	atrioventricular canal endocardium cell
Stage:	Long-pec

Fig. 5

Expression of Alcam in AV endocardial cells is expanded in id4 mutants. Alcam positive AV endocardial cells (yellow) were found to be increased in id4 mutants (B) as compared to their id4 heterozygous siblings (A) at 52 hpf. (C) Quantification of Alcam positive AV endocardial cells. (A, Atrium; AVC, Atrioventricular Canal; V, Ventricle; SAV, Superior AVC; IAV, Inferior AVC). Scale bars, 20 µm. p-value **- 0.0016, *- 0.0114. (Green – Alcam; Red - Tg(kdrl:ras-mCherry).

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
alcama	id4^bns18/bns18; s896Tg	control	Long-pec	atrioventricular canal endocardium cell	IHC
	s896Tg	control	Long-pec	atrioventricular canal endocardium cell	IHC
mCherry	id4^bns18/bns18; s896Tg	control	Long-pec	atrioventricular canal endocardium	IHC
	s896Tg	control	Long-pec	atrioventricular canal endocardium	IHC

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
id4^bns18/bns18; s896Tg	control	Long-pec	atrioventricular canal endocardium cell alcama expression increased distribution, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.