ZFIN ID: ZDB-FIG-160427-7
Ahuja et al., 2016 - Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development. Developmental Biology   412(1):71-82 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 6

Id4 mediates Bmp signaling leading to spp1 expression. Increased expression of id4 (A, B) and spp1 (C, D) was observed at 52 hpf in Tg(hsp70l:bmp2b) embryos after heat shock at 26 and 48 hpf for 30 min at 37 ºC. (E, F) spp1 expression was examined in id4 wild-type siblings and mutants in the Tg(hsp70l:bmp2b) background at 52 hpf after previously mentioned heat shock conditions. Unlike their id4+/+ siblings (E), most of the id4-/- (F) did not show an increase in spp1 expression after heat shock induced bmp2b overexpression. Scale bars, 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
id4 WT control Long-pec brain ISH
Long-pec heart ISH
fr13Tg heat shock Long-pec brain ISH
Long-pec heart ISH
spp1 WT control Long-pec brain ISH
Long-pec heart ISH
fr13Tg heat shock Long-pec brain ISH
Long-pec heart ISH
id4bns18/bns18; fr13Tg heat shock Long-pec brain ISH
Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fr13Tg heat shock Long-pec brain id4 expression increased amount, abnormal
Long-pec brain spp1 expression increased amount, abnormal
Long-pec heart id4 expression increased amount, abnormal
Long-pec heart spp1 expression increased amount, abnormal
id4bns18/bns18; fr13Tg heat shock Long-pec brain spp1 expression amount, ameliorated
Long-pec heart spp1 expression amount, ameliorated
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.