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ZFIN ID: ZDB-FIG-160427-9
Ahuja et al., 2016 - Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development. Developmental Biology   412(1):71-82 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 8

Id4 negatively regulates TCF function in the atrioventricular canal (AVC). (A, B, C) Larvae from incrosses of id4 heterozygous fish or from crossing id4+/- males to id4-/- females in a Tg(7xTCF-Xla.Siam:nlsmCherry) background were imaged at 77 hpf followed by quantification (D) of TCF positive nuclei. We observed a significant increase in the number of TCF reporter positive cells in id4 mutants as compared to siblings. (E, F) Transient endothelial specific overexpression of id4 caused a dose dependent reduction of TCF reporter signal (F) in the AVC as compared to the overexpression of GFP alone (E). Arrows point to GFP expressing TCF reporter positive nuclei (E) and id4 expressing TCF reporter negative nuclei (F). (G) Percentage of TCF reporter positive cells in transient GFP and id4 overexpression larvae and their intensity profile (H). (I, J) At 52 hpf, apc mutants show reduced spp1 expression as compared to their wild-type siblings. Scale bars, A-C and E-F: 10 µm; I-J: 100 µm (SAV, Superior AVC; IAV, Inferior AVC.) p value *<0.05, **<0.01, ***<0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry ia5Tg standard conditions Protruding-mouth endocardial cushion IFL
ia5Tg ; id4bns18/bns18 standard conditions Protruding-mouth endocardial cushion IFL
id4bns18/+ ; ia5Tg standard conditions Protruding-mouth endocardial cushion IFL
spp1 WT control Long-pec atrioventricular canal endocardium ISH
apchu745/hu745 control Long-pec atrioventricular canal endocardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apchu745/hu745 control Long-pec atrioventricular canal endocardium spp1 expression decreased amount, abnormal
ia5Tg ; id4bns18/bns18 standard conditions Protruding-mouth endocardial cushion mCherry expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.