ZFIN Figure: Ahuja et al., 2016, Fig. 4

ZFIN ID: ZDB-FIG-160427-5

ZFIN ID: ZDB-FIG-160427-5

Ahuja et al., 2016 - Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development. Developmental Biology 412(1):71-82 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	bmp4 has2 spp1 tbx2b vcana
Fish:	WT id4^bns18/bns18
Anatomical Terms:	atrioventricular canal myocardium atrioventricular canal endocardium atrium cardiac ventricle sinus venosus
Stage:	Long-pec

PHENOTYPE:

Fish:	id4^bns18/bns18
Observed In:	atrioventricular canal endocardium atrium cardiac ventricle sinus venosus
Stage:	Long-pec

Fig. 4

AV canal marker gene expression analysis in id4 mutants at 52 hpf. Myocardial (tbx2b, bmp4, cspg2a) and endocardial (has2, spp1) AVC marker gene expression was analyzed in embryos from incrosses of id4 heterozygous animals. After in situ hybridization, embryos were separated according to expression patterns and subsequently genotyped. Amongst these markers, tbx2b and has2 expression were found to be unchanged, bmp4 and cspg2a were misexpressed and spp1 expression was reduced in id4 mutants. Scale bars, 100 µm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
bmp4	WT	control	Long-pec	atrioventricular canal myocardium	ISH
	id4^bns18/bns18	standard conditions	Long-pec	atrioventricular canal myocardium	ISH
			Long-pec	cardiac ventricle	ISH
has2	WT	control	Long-pec	atrioventricular canal endocardium	ISH
	id4^bns18/bns18	standard conditions	Long-pec	atrioventricular canal endocardium	ISH
spp1	WT	control	Long-pec	atrioventricular canal endocardium	ISH
	id4^bns18/bns18	standard conditions	Long-pec	atrioventricular canal endocardium	ISH
tbx2b	WT	control	Long-pec	atrioventricular canal myocardium	ISH
	id4^bns18/bns18	standard conditions	Long-pec	atrioventricular canal myocardium	ISH
vcana	WT	control	Long-pec	atrioventricular canal myocardium	ISH
	id4^bns18/bns18	standard conditions	Long-pec	atrioventricular canal myocardium	ISH
			Long-pec	atrium	ISH
			Long-pec	sinus venosus	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
id4^bns18/bns18	standard conditions	Long-pec	atrioventricular canal endocardium spp1 expression decreased amount, abnormal
		Long-pec	atrium vcana expression mislocalised, abnormal
		Long-pec	cardiac ventricle bmp4 expression mislocalised, abnormal
		Long-pec	sinus venosus vcana expression mislocalised, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.