ZFIN ID: ZDB-FIG-160427-5
Ahuja et al., 2016 - Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development. Developmental Biology   412(1):71-82 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 4

AV canal marker gene expression analysis in id4 mutants at 52 hpf. Myocardial (tbx2b, bmp4, cspg2a) and endocardial (has2, spp1) AVC marker gene expression was analyzed in embryos from incrosses of id4 heterozygous animals. After in situ hybridization, embryos were separated according to expression patterns and subsequently genotyped. Amongst these markers, tbx2b and has2 expression were found to be unchanged, bmp4 and cspg2a were misexpressed and spp1 expression was reduced in id4 mutants. Scale bars, 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp4 WT control Long-pec atrioventricular canal myocardium ISH
id4bns18/bns18 standard conditions Long-pec atrioventricular canal myocardium ISH
Long-pec cardiac ventricle ISH
has2 WT control Long-pec atrioventricular canal endocardium ISH
id4bns18/bns18 standard conditions Long-pec atrioventricular canal endocardium ISH
spp1 WT control Long-pec atrioventricular canal endocardium ISH
id4bns18/bns18 standard conditions Long-pec atrioventricular canal endocardium ISH
tbx2b WT control Long-pec atrioventricular canal myocardium ISH
id4bns18/bns18 standard conditions Long-pec atrioventricular canal myocardium ISH
vcana WT control Long-pec atrioventricular canal myocardium ISH
id4bns18/bns18 standard conditions Long-pec atrioventricular canal myocardium ISH
Long-pec atrium ISH
Long-pec sinus venosus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
id4bns18/bns18 standard conditions Long-pec atrioventricular canal endocardium spp1 expression decreased amount, abnormal
Long-pec atrium vcana expression mislocalised, abnormal
Long-pec cardiac ventricle bmp4 expression mislocalised, abnormal
Long-pec sinus venosus vcana expression mislocalised, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.