ZFIN ID: ZDB-FIG-160427-2
Ahuja et al., 2016 - Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development. Developmental Biology   412(1):71-82 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 1

id4 expression analysis in zebrafish embryos and adult hearts. (A; A′) id4 is expressed in the brain and heart at 52 hpf. In the heart, expression is enriched in the AVC (red arrow) and inflow tract (black arrow). (B) Lack of id4 expression in clo mutant hearts suggests that id4 is expressed in the endocardium. (C; C′) In the adult heart, id4 is expressed in the atrium (black arrow) and AV canal (red arrow) exclusively. myh6 (atrial mhc) was used as a positive control for the atrium. (D) qPCR analysis of id4 expression in the adult zebrafish heart. (E) Section through adult heart after whole mount in situ hybridization. Arrows point to endocardial expression of id4 in the adult atrium and AVC. Scale bars, 100 ┬Ám. A, atrium; AVC, atrioventricular canal; V, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
id4 AB control Long-pec atrioventricular canal ISH
Long-pec brain ISH
Long-pec endocardium ISH
Long-pec heart ISH
Adult atrioventricular canal ISH
Adult atrium ISH
Adult atrium RTPCR
Adult Not Detected cardiac ventricle RTPCR
npas4ls5/s5 control Long-pec Not Detected endocardium ISH
Long-pec Not Detected heart ISH
myh6 AB control Adult atrium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
npas4ls5/s5 control Long-pec endocardium id4 expression absent, abnormal
Long-pec heart id4 expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.