ZFIN Figure: Ahuja et al., 2016, Fig. 1

ZFIN ID: ZDB-FIG-160427-2

ZFIN ID: ZDB-FIG-160427-2

Ahuja et al., 2016 - Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development. Developmental Biology 412(1):71-82 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	id4 myh6
Fish:	AB npas4l^s5/s5
Anatomical Terms:	atrioventricular canal atrium brain endocardium heart
Stage Range:	Long-pec to Adult

PHENOTYPE:

Fish:	npas4l^s5/s5
Observed In:	endocardium heart
Stage:	Long-pec

Fig. 1

id4 expression analysis in zebrafish embryos and adult hearts. (A; A′) id4 is expressed in the brain and heart at 52 hpf. In the heart, expression is enriched in the AVC (red arrow) and inflow tract (black arrow). (B) Lack of id4 expression in clo mutant hearts suggests that id4 is expressed in the endocardium. (C; C′) In the adult heart, id4 is expressed in the atrium (black arrow) and AV canal (red arrow) exclusively. myh6 (atrial mhc) was used as a positive control for the atrium. (D) qPCR analysis of id4 expression in the adult zebrafish heart. (E) Section through adult heart after whole mount in situ hybridization. Arrows point to endocardial expression of id4 in the adult atrium and AVC. Scale bars, 100 µm. A, atrium; AVC, atrioventricular canal; V, ventricle.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
id4	AB	control	Long-pec		atrioventricular canal	ISH
			Long-pec		brain	ISH
			Long-pec		endocardium	ISH
			Long-pec		heart	ISH
			Adult		atrioventricular canal	ISH
			Adult		atrium	ISH
			Adult		atrium	RTPCR
			Adult	Not Detected	cardiac ventricle	RTPCR
	npas4l^s5/s5	control	Long-pec	Not Detected	endocardium	ISH
			Long-pec	Not Detected	heart	ISH
myh6	AB	control	Adult		atrium	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
npas4l^s5/s5	control	Long-pec	endocardium id4 expression absent, abnormal
		Long-pec	heart id4 expression absent, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.