ZFIN ID: ZDB-IMAGE-160427-1
Figures for Ahuja et al., 2016

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Fig. 1

id4 expression analysis in zebrafish embryos and adult hearts. (A; A′) id4 is expressed in the brain and heart at 52 hpf. In the heart, expression is enriched in the AVC (red arrow) and inflow tract (black arrow). (B) Lack of id4 expression in clo mutant hearts suggests that id4 is expressed in the endocardium. (C; C′) In the adult heart, id4 is expressed in the atrium (black arrow) and AV canal (red arrow) exclusively. myh6 (atrial mhc) was used as a positive control for the atrium. (D) qPCR analysis of id4 expression in the adult zebrafish heart. (E) Section through adult heart after whole mount in situ hybridization. Arrows point to endocardial expression of id4 in the adult atrium and AVC. Scale bars, 100 ┬Ám. A, atrium; AVC, atrioventricular canal; V, ventricle.

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Reprinted from Developmental Biology, 412(1), Ahuja, S., Dogra, D., Stainier, D.Y., Reischauer, S., Id4 functions downstream of Bmp signaling to restrict TCF function in endocardial cells during atrioventricular valve development, 71-82, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.