ZFIN ID: ZDB-FIG-150402-2
Kok et al., 2015 - Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish. Developmental Cell   32(1):97-108 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 2

Normal ISV Development in Selected Mutant Embryos

(A–F) Confocal micrographs of Tg(fli1a:egfp)y1 or Tg(kdrl:egfp)s843 transgenic embryos at 32 hrpf subjected to immunostaining with GFP antibody. (A) Wild-type sibling: ISVs, DLAV, and DA are indicated. (B–F) Embryos mutant for (B) arhgef9bum45, (C) fgd5aum134, (D) kalrnbum80, (E) fmnl3um150, or (F) pdgfrbum148. Lateral views, anterior to the left, and dorsal is up.

(G) Whole mount in situ hybridization analysis of fmnl3 wild-type and fmnl3um150 mutant sibling at 26 hrpf. Lateral views, anterior to the left, and dorsal is up.

(H) Schematic of fmnl3 locus flanking um150 allele and genotyping of individual embryo heads at 72 hrpf from an in-cross of fmnl3um150 heterozygous carriers. Homozygous mutants and heterozygotes pooled for subsequent RT-PCR are indicated by M and het, respectively.

(I) Schematic of fmnl3 transcript flanking um150 and RT-PCR analysis of trunks from pooled M and het embryos shown in (H).

(J) Whole mount in situ hybridization analysis of pdgfrb in wild-type and pdgfrbum148 mutant at 72 hrpf. Lateral views, anterior to the left, and dorsal is up.

(K) Western blot analysis of Pdgfrb and Egfp in lysates from Tg(fli1a:egfp)y1;pdgfrbum148 of indicated genotype at 72 hrpf.

(L) Schematic of ets1 exon 3 bearing the um206 lesion and genotyping of individual embryo heads at 3 days postfertilization from an in-cross of ets1um206 heterozygous carriers.

(M) Schematic of ets1 transcript and RT-PCR analysis of trunks from pooled M and het embryos shown in (L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fmnl3 WT standard conditions Prim-5 anatomical structure ISH
fmnl3um150/um150 standard conditions Prim-5 anatomical structure ISH
pdgfrb WT standard conditions Protruding-mouth anatomical structure ISH
Ab1-pdgfrb pdgfrbum148/+ ; y1Tg standard conditions Protruding-mouth whole organism WB
pdgfrbum148/um148 standard conditions Protruding-mouth anatomical structure ISH
Ab1-pdgfrb pdgfrbum148/um148 ; y1Tg standard conditions Protruding-mouth Not Detected whole organism WB
Ab1-pdgfrb y1Tg standard conditions Protruding-mouth whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgd5aum134/um134 ; s843Tg standard conditions Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
fmnl3um150/um150 standard conditions Prim-5 anatomical structure fmnl3 expression decreased distribution, abnormal
fmnl3um150/um150 ; y1Tg standard conditions Prim-15 intersegmental vessel morphology, normal
Prim-15 intersegmental vessel present, normal
Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
kalrnbum80/um80; s843Tg standard conditions Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
kalrnbum80/um80; y1Tg standard conditions Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
pdgfrbum148/+ ; y1Tg standard conditions Protruding-mouth whole organism pdgfrb expression decreased amount, abnormal
pdgfrbum148/um148 standard conditions Protruding-mouth anatomical structure pdgfrb expression decreased distribution, abnormal
pdgfrbum148/um148 ; y1Tg standard conditions Prim-15 intersegmental vessel morphology, normal
Prim-15 intersegmental vessel present, normal
Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
Protruding-mouth whole organism pdgfrb expression absent, abnormal
s843Tg ; arhgef9bum45/um45 standard conditions Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
s843Tg ; fmnl3um150/um150 standard conditions Prim-15 intersegmental vessel morphology, normal
Prim-15 intersegmental vessel present, normal
Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
s843Tg ; pdgfrbum148/um148 standard conditions Prim-15 intersegmental vessel morphology, normal
Prim-15 intersegmental vessel present, normal
Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
y1Tg ; arhgef9bum45/um45 standard conditions Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
y1Tg ; fgd5aum134/um134 standard conditions Prim-15 vasculature morphology, normal
Prim-15 vasculature development process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 32(1), Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D., Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish, 97-108, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell