ZFIN Figure: Kok et al., 2015, Fig. 5

ZFIN ID: ZDB-FIG-150402-5

ZFIN ID: ZDB-FIG-150402-5

Kok et al., 2015 - Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish. Developmental Cell 32(1):97-108 Full text @ Dev. Cell

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	birc6-as2 eef1a1l1 oip5-as1
Fish:	WT birc6-as2^um209/um209
Anatomical Term:	whole organism
Stage:	Long-pec

PHENOTYPE:

Fish:	birc6-as2^um209/um209 birc6-as2^um209/um209 + MO3-birc6-as2 WT + MO3-birc6-as2
Knockdown Reagent:	MO3-birc6-as2
Observed In:	fourth ventricle whole organism
Stage:	Long-pec

Fig. 5

Normal Hindbrain Development in megamind Mutant Embryos

(A) Schematic of the megamind locus in wild-type and megamind^um209 mutants. The red and green boxes indicate relative position of the TALEN target sequences, and the MO target sequence is shown.

(B) RT-PCR for the megamind and cyrano lincRNAs as well as eef1al1 in wild-type and megamind^um209 mutant embryos. RT refers to RNA template without () or with (+) reverse transcription.

(C–F) Transmitted light images of the head region in embryos at 48 hrpf. The hindbrain ventricle is indicated with an arrow in each image. Lateral views, anterior to the left, and dorsal is up. (C) Wild-type, uninjected; (D) megamind^um209 mutant; (E) wild-type injected with 20 ng megamind conserved site MO; and (F) megamind^um209 mutant injected with megamind conserved site MO.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
birc6-as2	WT	standard conditions	Long-pec		whole organism	RTPCR
	birc6-as2^um209/um209	standard conditions	Long-pec	Not Detected	whole organism	RTPCR
eef1a1l1	WT	standard conditions	Long-pec		whole organism	RTPCR
	birc6-as2^um209/um209	standard conditions	Long-pec		whole organism	RTPCR
oip5-as1	WT	standard conditions	Long-pec		whole organism	RTPCR
	birc6-as2^um209/um209	standard conditions	Long-pec		whole organism	RTPCR

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT + MO3-birc6-as2	standard conditions	Long-pec	fourth ventricle hydrocephalic, abnormal
birc6-as2^um209/um209	standard conditions	Long-pec	fourth ventricle structure, normal
		Long-pec	whole organism birc6-as2 expression absent, abnormal
birc6-as2^um209/um209 + MO3-birc6-as2	standard conditions	Long-pec	fourth ventricle hydrocephalic, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Cell, 32(1), Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D., Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish, 97-108, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 32(1), Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D., Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish, 97-108, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell