ZFIN ID: ZDB-FIG-150402-5
Kok et al., 2015 - Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish. Developmental Cell   32(1):97-108 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5

Normal Hindbrain Development in megamind Mutant Embryos

(A) Schematic of the megamind locus in wild-type and megamindum209 mutants. The red and green boxes indicate relative position of the TALEN target sequences, and the MO target sequence is shown.

(B) RT-PCR for the megamind and cyrano lincRNAs as well as eef1al1 in wild-type and megamindum209 mutant embryos. RT refers to RNA template without () or with (+) reverse transcription.

(C–F) Transmitted light images of the head region in embryos at 48 hrpf. The hindbrain ventricle is indicated with an arrow in each image. Lateral views, anterior to the left, and dorsal is up. (C) Wild-type, uninjected; (D) megamindum209 mutant; (E) wild-type injected with 20 ng megamind conserved site MO; and (F) megamindum209 mutant injected with megamind conserved site MO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
birc6-as2 WT standard conditions Long-pec whole organism RTPCR
birc6-as2um209/um209 standard conditions Long-pec Not Detected whole organism RTPCR
eef1a1l1 WT standard conditions Long-pec whole organism RTPCR
birc6-as2um209/um209 standard conditions Long-pec whole organism RTPCR
oip5-as1 WT standard conditions Long-pec whole organism RTPCR
birc6-as2um209/um209 standard conditions Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-birc6-as2 standard conditions Long-pec fourth ventricle hydrocephalic, abnormal
birc6-as2um209/um209 standard conditions Long-pec fourth ventricle structure, normal
Long-pec whole organism birc6-as2 expression absent, abnormal
birc6-as2um209/um209 + MO3-birc6-as2 standard conditions Long-pec fourth ventricle hydrocephalic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 32(1), Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., Lawson, N.D., Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish, 97-108, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell