ZFIN ID: ZDB-FIG-150213-3
Paik et al., 2013 - A cdx4-sall4 regulatory module controls the transition from mesoderm formation to embryonic hematopoiesis. Stem Cell Reports   1(5):425-436 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 10-13 somites

Fig. 4

scl and lmo2 Are Responsible for the Loss of RBCs Seen in cdx4-/-;sall4mo Embryos.

(A and B) scl (A) and lmo2 (B) WISH at the 10-somite stage wild-type or cdx4-/- embryos that are either uninjected or sall4mo injected. Embryos were flat-mounted, with the anterior end pointing to the left and the posterior end pointing to the right.

(C) fli1a, draculin, pax2a, myoD, and tbx16 WISH in 10-somite stage wild-type, cdx4mo, sall4mo, cdx4mo/sall4mo embryos. All scale bars, 200 ┬Ám.

(D and E) Gene track of the scl (D) and lmo2 (E) loci, showing Cdx4 and Sall4 binding.

See also Figure S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
drl WT standard conditions 10-13 somites lateral plate mesoderm ISH
WT + MO1-cdx4 standard conditions 10-13 somites lateral plate mesoderm ISH
WT + MO1-cdx4 + MO1-sall4 standard conditions 10-13 somites lateral plate mesoderm ISH
WT + MO1-sall4 standard conditions 10-13 somites lateral plate mesoderm ISH
fli1 WT standard conditions 10-13 somites lateral plate mesoderm ISH
WT + MO1-cdx4 standard conditions 10-13 somites lateral plate mesoderm ISH
WT + MO1-cdx4 + MO1-sall4 standard conditions 10-13 somites lateral plate mesoderm ISH
WT + MO1-sall4 standard conditions 10-13 somites lateral plate mesoderm ISH
lmo2 WT standard conditions 10-13 somites lateral plate mesoderm ISH
WT + MO1-sall4 standard conditions 10-13 somites lateral plate mesoderm ISH
cdx4tv205c/tv205c standard conditions 10-13 somites lateral plate mesoderm ISH
cdx4tv205c/tv205c + MO1-sall4 standard conditions 10-13 somites lateral plate mesoderm ISH
myod1 WT standard conditions 10-13 somites somite ISH
WT + MO1-cdx4 standard conditions 10-13 somites somite ISH
WT + MO1-cdx4 + MO1-sall4 standard conditions 10-13 somites somite ISH
WT + MO1-sall4 standard conditions 10-13 somites somite ISH
pax2a WT standard conditions 10-13 somites pronephros ISH
WT + MO1-cdx4 standard conditions 10-13 somites pronephros ISH
WT + MO1-cdx4 + MO1-sall4 standard conditions 10-13 somites pronephros ISH
WT + MO1-sall4 standard conditions 10-13 somites pronephros ISH
tal1 WT standard conditions 10-13 somites lateral plate mesoderm ISH
WT + MO1-sall4 standard conditions 10-13 somites lateral plate mesoderm ISH
cdx4tv205c/tv205c standard conditions 10-13 somites lateral plate mesoderm ISH
cdx4tv205c/tv205c + MO1-sall4 standard conditions 10-13 somites lateral plate mesoderm ISH
tbx16 WT standard conditions 10-13 somites segmental plate ISH
WT + MO1-cdx4 standard conditions 10-13 somites segmental plate ISH
WT + MO1-cdx4 + MO1-sall4 standard conditions 10-13 somites segmental plate ISH
WT + MO1-sall4 standard conditions 10-13 somites segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports