ZFIN ID: ZDB-FIG-140709-41
Feng et al., 2010 - Dhrs3a regulates retinoic acid biosynthesis through a feedback inhibition mechanism. Developmental Biology   338(1):1-14 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 1-4 somites

Fig. 8 Dhrs3a knockdown effects nervous system patterning. (A–C) in situ hybridization of hoxd4a (blue), pax2a (red in mid-hindbrain boundary, otic vesicles and somites) and egr2b (red in rhombomere 3 and 5) in wt (A), dhrs3a MO1 (B) and MO3 (C)-injected embryos. Dotted arrows indicate the distance from the r6/7 boundary, marked by the anterior limit of hoxd4a expression, to the second somite (indicated by the anterior limit of pax2a expression). Solid arrows indicate the distance from the r3/4 boundary to the second somite. Both distances are longer in dhrs3a knockdown embryos. (D–G) Knockdown of Dhrs3 enhances the posteriorized hindbrain phenotype of cyp26a1 mutant embryos: (D) wt; (E) cyp26a1-/gir mutant; (F, G) cyp26a1-/gir mutant injected with dhrs3a MO3 (F) or MO1 (G).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b AB standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
AB + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
AB + MO2-dhrs3a + MO4-tp53 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
cyp26a1rw716/rw716 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
cyp26a1rw716/rw716 + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
cyp26a1rw716/rw716 + MO2-dhrs3a + MO4-tp53 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
hoxd4a AB standard conditions 1-4 somites hindbrain neural plate ISH
AB + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites hindbrain neural plate ISH
AB + MO2-dhrs3a + MO4-tp53 standard conditions 1-4 somites hindbrain neural plate ISH
cyp26a1rw716/rw716 standard conditions 1-4 somites hindbrain neural plate ISH
cyp26a1rw716/rw716 + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites hindbrain neural plate ISH
cyp26a1rw716/rw716 + MO2-dhrs3a + MO4-tp53 standard conditions 1-4 somites hindbrain neural plate ISH
pax2a WT standard conditions 1-4 somites midbrain hindbrain boundary neural plate ISH
1-4 somites otic placode ISH
1-4 somites somite ISH
WT + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites midbrain hindbrain boundary neural plate ISH
1-4 somites otic placode ISH
1-4 somites somite ISH
WT + MO2-dhrs3a + MO4-tp53 standard conditions 1-4 somites midbrain hindbrain boundary neural plate ISH
1-4 somites otic placode ISH
1-4 somites somite ISH
cyp26a1rw716/rw716 standard conditions 1-4 somites midbrain hindbrain boundary neural plate ISH
1-4 somites otic placode ISH
1-4 somites somite ISH
cyp26a1rw716/rw716 + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites midbrain hindbrain boundary neural plate ISH
1-4 somites otic placode ISH
1-4 somites somite ISH
cyp26a1rw716/rw716 + MO2-dhrs3a + MO4-tp53 standard conditions 1-4 somites midbrain hindbrain boundary neural plate ISH
1-4 somites otic placode ISH
1-4 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 338(1), Feng, L., Hernandez, R.E., Waxman, J.S., Yelon, D., and Moens, C.B., Dhrs3a regulates retinoic acid biosynthesis through a feedback inhibition mechanism, 1-14, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.