ZFIN ID: ZDB-FIG-140709-42
Feng et al., 2010 - Dhrs3a regulates retinoic acid biosynthesis through a feedback inhibition mechanism. Developmental Biology   338(1):1-14 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 1-4 somites

Fig. 9 Dhrs3 knockdown effects hindbrain neuronal organization. (A–D) RMO44 immunostaining of hindbrain reticulospinal neurons: (A) control wt embryo with 5 ng tp53 MO; (B) dhrs3a MO1+tp53 MO-injected embryo; (C) cyp26a1 mutant embryo injected with 5 ng control tp53 MO; (D) cyp26a1 mutant embryo injected with dhrs3a MO1+tp53 MO. Arrows indicate the Mauthner neuron in r4, which is unaffected under all conditions; arrowheads indicate the distinctive T-interneurons which lie in r7 and anterior r8. (E) Quantification of the number of T-interneurons in each condition. N refers to the number of embryos counted.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-neurofilament-m IHC AB + MO4-tp53 standard conditions 1-4 somites hindbrain interneuron
IHC 1-4 somites T interneuron
IHC WT + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites hindbrain interneuron
IHC 1-4 somites T interneuron
IHC cyp26a1rw716/rw716 + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites hindbrain interneuron
IHC 1-4 somites T interneuron
IHC cyp26a1rw716/rw716 + MO4-tp53 standard conditions 1-4 somites hindbrain interneuron
IHC 1-4 somites T interneuron
Phenotype Details
Fish Conditions Stage Phenotype
cyp26a1rw716/rw716 + MO1-dhrs3a + MO4-tp53 standard conditions 1-4 somites hindbrain has fewer parts of type T interneuron, abnormal
cyp26a1rw716/rw716 + MO4-tp53 standard conditions 1-4 somites hindbrain has fewer parts of type T interneuron, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 338(1), Feng, L., Hernandez, R.E., Waxman, J.S., Yelon, D., and Moens, C.B., Dhrs3a regulates retinoic acid biosynthesis through a feedback inhibition mechanism, 1-14, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.