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ZFIN ID: ZDB-FIG-140522-43
Ning et al., 2013 - MicroRNA-92a upholds Bmp signaling by targeting noggin3 during pharyngeal cartilage formation. Developmental Cell   24(3):283-295 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: High-pec

Fig. S5 Test of mir92a targets, related to Figure 5. (A) Schematic diagrams of reporter constructs. (B) The expression of the reporter GFP mRNA was unaffected by mir92a duplex. 60 pg of GFP mRNA was injected together with 1 nl of 20 M Ctrl duplex or mir92a duplex at the one-cell stage, and the injected embryos were observed for GFP fluorescence at 24 hpf. Scale bars, 1 mm. (C-E) mir92a had no effect on the expression of GFP-nog3-3′UTR (C), GFP-smad6a-3′UTR (D) and GFP-smad7-3′UTR (E) mRNAs. 60 pg of each mRNA species were co-injected with 1 nl of 20 M Ctrl duplex or mir92a duplex at the one-cell stage. The injected embryos were observed at 24 hpf. Scale bars, 1 mm. (F) bmp2b and bmp4 expression was detected by whole-mount in situ hybridization in uninjected control embryos (UIC) or mir92a morphants at 42 hpf. (G) Tg(fli1:GFP) transgenic embryos at 42 hpf were immunostained with anti-GFP and anti-Zn-5 antibodies. Scale bars, 50 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp2b WT standard conditions High-pec pharyngeal pouch ISH
WT + MO2-mir92a standard conditions High-pec pharyngeal pouch ISH
bmp4 WT standard conditions High-pec pharyngeal pouch ISH
WT + MO2-mir92a standard conditions High-pec pharyngeal pouch ISH
EGFP y1Tg standard conditions High-pec pharyngeal arch ISH
y1Tg + MO2-mir92a standard conditions High-pec pharyngeal arch IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC y1Tg standard conditions High-pec pharyngeal pouch
IHC y1Tg + MO2-mir92a standard conditions High-pec pharyngeal pouch
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 24(3), Ning, G., Liu, X., Dai, M., Meng, A., and Wang, Q., MicroRNA-92a upholds Bmp signaling by targeting noggin3 during pharyngeal cartilage formation, 283-295, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell